Zhengzhou Research Base, State Key Laboratory of Cotton Biology, School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450052, People's Republic of China; Henan Key Laboratory of Ion-Beam Bioengineering, Zhengzhou University, Zhengzhou 450052, People's Republic of China.
Henan Key Laboratory of Ion-Beam Bioengineering, Zhengzhou University, Zhengzhou 450052, People's Republic of China.
Ecotoxicol Environ Saf. 2022 Jul 15;240:113703. doi: 10.1016/j.ecoenv.2022.113703. Epub 2022 May 31.
Atmospheric pressure cold plasma (APCP) holds great potential as an efficient, economical and eco-friendly approach for improving crop production. Although APCP-induced plant growth promotion is undisputedly attributed to the reactive oxygen and nitrogen species (RONS), how these RONS regulate the intracellular redox state and plant growth is still largely unknown. This study systematically investigates the regulation mechanism of APCP-generated RONS on intracellular redox homeostasis in Arabidopsis thaliana seedling by measuring the RONS compositions in APCP-treated solutions and intracellular RONS and antioxidants in Arabidopsis seedlings. The results show that APCP exhibited a dual effect (stimulation or inhibition) on Arabidopsis seedling growth dependent on the treatment time. APCP-generated RONS in liquids increased in a time-dependent manner, leading to an increase of conductivity and oxidation reduction potential (ORP) and decrease of pH. APCP caused an enrichment of intracellular RONS and most of them increased with APCP treatment time. Meanwhile, APCP treatment accelerated malondialdehyde (MDA) generation, and the level of intracellular antioxidants were enhanced by low-dose APCP treatment while decreased at high doses. The results of correlation analysis showed that the extracellular RONS produced by APCP were positively correlated with the intracellular RONS and negatively correlated with the antioxidants. These results demonstrate that the improved antioxidant capacity induced by moderate APCP-generated RONS plays an important role in the growth promotion of Arabidopsis seedlings, which may be a promising alternative for fertilizers in agricultural production.
大气压冷等离子体（APCP）作为一种高效、经济和环保的方法，具有提高作物产量的巨大潜力。尽管 APPC 诱导的植物生长促进作用无可争议地归因于活性氧和氮物种（RONS），但这些 RONS 如何调节细胞内氧化还原状态和植物生长仍在很大程度上未知。本研究通过测量 APCP 处理溶液中的 RONS 组成和拟南芥幼苗中的细胞内 RONS 和抗氧化剂，系统研究了 APCP 产生的 RONS 对拟南芥幼苗细胞内氧化还原平衡的调节机制。结果表明，APCP 对拟南芥幼苗生长表现出双重作用（刺激或抑制），取决于处理时间。液体中 APCP 产生的 RONS 随时间呈时间依赖性增加，导致电导率和氧化还原电位（ORP）增加，pH 值降低。APCP 导致细胞内 RONS 富集，其中大多数随着 APCP 处理时间的增加而增加。同时，APCP 处理加速丙二醛（MDA）的生成，低剂量 APCP 处理增强细胞内抗氧化剂水平，而高剂量则降低。相关分析的结果表明，APCP 产生的细胞外 RONS 与细胞内 RONS 呈正相关，与抗氧化剂呈负相关。这些结果表明，适度的 APCP 产生的 RONS 诱导的抗氧化能力的提高在拟南芥幼苗的生长促进中起着重要作用，这可能是农业生产中肥料的一种有前途的替代品。
