Biological Materials Laboratory, CSIR-Central Leather Research Institute, Chennai, Tamil Nadu 600020, India; University of Madras, Chennai, Tamil Nadu 600005, India.
Biological Materials Laboratory, CSIR-Central Leather Research Institute, Chennai, Tamil Nadu 600020, India.
Colloids Surf B Biointerfaces. 2022 Aug;216:112589. doi: 10.1016/j.colsurfb.2022.112589. Epub 2022 May 21.
Rare earth lanthanum oxide nanoparticle reinforced collagen biomatrix that elicited the endothelial cell activation to promote angiogenesis for biomaterial integration was developed and evaluated in the present study. The structural integrity of collagen was not compromised on crosslinking of lanthanum oxide nanoparticle to collagen biomolecule. As-synthesised collagen biomatrix was shown to have improved mechanical strength, a lesser susceptibility to proteolytic degradation and good swelling properties. Superior cytocompatibility, hemocompatibility and minimal ROS generation was observed with Lanthanum oxide nanoparticle reinforced collagen bio matrix. The Lanthanum oxide nanoparticle reinforced collagen bio matrix elicited endothelial cell activation eliciting pro-angiogensis as observed in tube formation and aortic arch assays. The bio-matrix promoted the infiltration and proliferation of endothelial cells which is an unexplored domain in the area of tissue engineering that is very essential for biomaterial integration into host tissue. The wound healing effect of Lanthanum oxide nanoparticle stabilized collagen showed enhanced cell migration in vitro in cells maintained in Lanthanum oxide nanoparticle reinforced collagen bio matrix. The study paves the way for developing rare earth-based dressing materials which promoted biomatrix integration by enhancing vascularisation for tissue regenerative applications in comparison with traditional biomaterials.
本研究开发并评价了一种基于稀土氧化镧纳米粒子增强胶原生物基质的方法，该生物基质可激活内皮细胞，促进血管生成，从而实现生物材料的整合。氧化镧纳米粒子与胶原生物分子交联不会破坏胶原的结构完整性。结果表明，合成的胶原生物基质具有更好的机械强度、较低的蛋白水解降解易感性和良好的溶胀性能。与氧化镧纳米粒子增强胶原生物基质相比，观察到其具有更好的细胞相容性、血液相容性和最小的 ROS 生成。氧化镧纳米粒子增强胶原生物基质可激活内皮细胞，促进血管生成，如管形成和主动脉弓试验所示。生物基质促进了内皮细胞的浸润和增殖，这是组织工程领域中一个尚未探索的领域，对于生物材料整合到宿主组织中非常重要。氧化镧纳米粒子稳定胶原的伤口愈合效果显示，在维持于氧化镧纳米粒子增强胶原生物基质中的细胞中，体外细胞迁移得到增强。该研究为开发基于稀土的敷料材料铺平了道路，与传统生物材料相比，这些敷料材料通过增强血管生成来促进生物基质整合，从而可应用于组织再生。
