Department of General Surgery, Xiangya Hospital, Central South University (CSU), Changsha, Hunan, China; State Key Laboratory of Applied Organic Chemistry and College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou, Gansu, 730000, China; Department of Molecular Medicine, The Scripps Research Institute, Jupiter, FL, USA.
State Key Laboratory of Applied Organic Chemistry and College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou, Gansu, 730000, China.
Arch Biochem Biophys. 2022 Sep 30;727:109303. doi: 10.1016/j.abb.2022.109303. Epub 2022 May 31.
Natural products derived from the daily diet are garnering increasing attention for neurodegenerative disease (ND) treatment. Hispolon (His), a small molecule from Phellinus linteus, has been reported to have various pharmacological activities. Here, we evaluated its protective effect on a neuron-like rat pheochromocytoma cell line (PC12). Results showed that His could restore cell death induced by oxidative damage. Nuclear factor-erythroid 2 (NF-E2)-related factor 2 (Nrf2) plays a significant role in maintaining cellular redox homeostasis. After treatment with His, some Nrf2-governed antioxidant genes were upregulated in a dose-dependent manner. However, the protective effect of His on PC12 cells was easily terminated by Nrf2 knockdown, demonstrating that Nrf2 is a critical component in this cytoprotective process. Taken together, our study showed that His was not only an effective activator of Nrf2 but also a promising candidate for ND treatment.
天然产物来源于日常饮食，对于治疗神经退行性疾病（ND）越来越受到关注。云芝酮（His）是来自云芝的一种小分子化合物，据报道具有多种药理活性。在这里，我们评估了它对神经元样大鼠嗜铬细胞瘤细胞系（PC12）的保护作用。结果表明，His 可以恢复由氧化损伤引起的细胞死亡。核因子-红细胞 2（NF-E2）相关因子 2（Nrf2）在维持细胞氧化还原平衡中起着重要作用。用 His 处理后，一些 Nrf2 调控的抗氧化基因呈剂量依赖性地上调。然而，His 对 PC12 细胞的保护作用很容易被 Nrf2 敲低所终止，表明 Nrf2 是这种细胞保护过程中的关键组成部分。总之，我们的研究表明，His 不仅是 Nrf2 的有效激活剂，也是治疗 ND 的有前途的候选药物。
