Liu Yi-Shan, Lai Mei-Chou, Tzeng Yu-Cheng, Liu I-Min
Department of Dermatology, E-Da Hospital, I-Shou University, Kaohsiung City 84001, Taiwan.
School of Chinese Medicine for Post Baccalaureate, College of Medicine, I-Shou University, Kaohsiung City 84001, Taiwan.
Nutrients. 2025 Jan 13;17(2):266. doi: 10.3390/nu17020266.
: This study investigated the wound-healing potential of hispolon, a polyphenolic pigment derived from medicinal mushrooms, under diabetic conditions using both in vitro and in vivo models. : In the in vitro assays, L929 fibroblast cells exposed to high glucose (33 mmol/L) were treated with hispolon at concentrations of 2.5, 5, 7.5, or 10 μmol/L. In the in vivo assays, streptozotocin-induced diabetic rats with excision wounds received daily topical applications of 0.2 g of 5% (/) hispolon ointment. : Hispolon improved cell viability; suppressed oxidative stress by reducing reactive oxygen species, lipid peroxidation, and oxidative DNA damage; and restored the reduced glutathione/oxidized glutathione ratio. The scratch assay demonstrated that hispolon at 10 μmol/L enhanced fibroblast migration impaired by high-glucose conditions. Treatment with 5% (/) hispolon ointment accelerated wound contraction, reduced the epithelialization time, and enhanced tissue regeneration with an efficacy comparable to that of Fespixon cream, as shown by histological findings of increased fibroblast activity, collagen deposition, and capillary growth. Hispolon ointment also modulated macrophage polarization in diabetic wounds by reducing M1 markers and enhancing M2 markers. In a diabetic rat dead-space-wound model, 5% (/) hispolon ointment reduced the levels of pro-inflammatory cytokines, increased those of anti-inflammatory cytokines and growth factors, and stimulated Type I and III collagen synthesis, effectively promoting wound healing. In incisional wounds, hispolon ointment improved the wound-breaking strength, showing results comparable to that of Fespixon cream. Safety assessments confirmed that hispolon ointment showed no acute dermal toxicity. These findings underscore hispolon's potential as a promising candidate for diabetic wound management by mitigating oxidative stress, enhancing tissue regeneration, and accelerating wound healing.
本研究使用体外和体内模型，研究了从药用蘑菇中提取的多酚类色素白蛇根素在糖尿病条件下的伤口愈合潜力。在体外试验中，将暴露于高糖（33 mmol/L）的L929成纤维细胞用浓度为2.5、5、7.5或10 μmol/L的白蛇根素处理。在体内试验中，链脲佐菌素诱导的有切除伤口的糖尿病大鼠每天局部应用0.2 g 5%（/）的白蛇根素软膏。白蛇根素提高了细胞活力；通过减少活性氧、脂质过氧化和氧化性DNA损伤来抑制氧化应激；并恢复了还原型谷胱甘肽/氧化型谷胱甘肽的比例。划痕试验表明，10 μmol/L的白蛇根素增强了受高糖条件损害的成纤维细胞迁移。如成纤维细胞活性增加、胶原蛋白沉积和毛细血管生长的组织学结果所示，用5%（/）的白蛇根素软膏治疗可加速伤口收缩，缩短上皮化时间，并增强组织再生，其疗效与非司匹克松乳膏相当。白蛇根素软膏还通过减少M1标志物和增强M2标志物来调节糖尿病伤口中的巨噬细胞极化。在糖尿病大鼠死腔伤口模型中，5%（/）的白蛇根素软膏降低了促炎细胞因子水平，增加了抗炎细胞因子和生长因子水平，并刺激了I型和III型胶原蛋白合成，有效促进了伤口愈合。在切口伤口中，白蛇根素软膏提高了伤口抗张强度，结果与非司匹克松乳膏相当。安全性评估证实，白蛇根素软膏没有急性皮肤毒性。这些发现强调了白蛇根素通过减轻氧化应激、增强组织再生和加速伤口愈合，作为糖尿病伤口管理有前景候选药物的潜力。
