Shaanxi Key Laboratory of Environmental Engineering, Key Laboratory of Northwest Water Resource, Environment and Ecology, MOE, Xi'an University of Architecture and Technology, Xi'an 710055, China; School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China.
Shaanxi Key Laboratory of Environmental Engineering, Key Laboratory of Northwest Water Resource, Environment and Ecology, MOE, Xi'an University of Architecture and Technology, Xi'an 710055, China; School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China.
Sci Total Environ. 2022 Sep 10;838(Pt 3):156475. doi: 10.1016/j.scitotenv.2022.156475. Epub 2022 Jun 2.
Less attention has been paid on the oligotrophic water body nitrogen treatment with mix-cultured aerobic denitrifying bacteria (Mix-CADB). In this study, three Mix-CADB communities were screened from the sediments of reservoirs. The nitrate and dissolved organic carbon (DOC) removal efficiencies of Mix-CADB communities were higher than 92 % and 91 %, respectively. Biolog results suggested that Mix-CADB communities displayed excellent carbon source metabolic activity. The nirS gene sequencing indicated that Pseudomonas sp. and Pseudomonas stutzeri accounted for more proportions in the core species of three Mix-CADB communities. The network model revealed that Pseudomonas sp. and Pseudomonas stutzeri mainly drove the total nitrogen and DOC removal of Mix-CADB communities. More importantly, the immobilized Mix-CADB communities could reduce >91 % nitrate in the adjusted reservoir raw water. Overall, this study showed that the three Mix-CADB communities could be regarded as potential candidates for the nitrogen treatment in oligotrophic water body ecosystems.
对于贫营养水体的氮处理，混合好氧反硝化细菌（Mix-CADB）的关注度较低。本研究从水库沉积物中筛选出三种 Mix-CADB 群落。Mix-CADB 群落对硝酸盐和溶解有机碳（DOC）的去除效率均高于 92%和 91%。Biolog 结果表明，Mix-CADB 群落表现出优异的碳源代谢活性。nirS 基因测序结果表明，假单胞菌和施氏假单胞菌在三种 Mix-CADB 群落的核心种中占比较大。网络模型揭示了假单胞菌和施氏假单胞菌主要驱动 Mix-CADB 群落的总氮和 DOC 去除。更重要的是，固定化的 Mix-CADB 群落可以减少调整后的水库原水中超过 91%的硝酸盐。总体而言，本研究表明，这三种 Mix-CADB 群落可作为贫营养水体生态系统中氮处理的潜在候选物。
