Department of Biology, College of Science, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh, 11671, Saudi Arabia.
Department of Biology, College of Science, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh, 11671, Saudi Arabia.
Plant Physiol Biochem. 2022 Aug 15;185:45-54. doi: 10.1016/j.plaphy.2022.05.029. Epub 2022 May 27.
The elevated level of CO (eCO) and arbuscular mycorrhizal fungi (AMF) have been known as successful eco-friendly agents for plant growth and development as well as quality enhancers. The current investigation was designed to study the influence of eCO (620 μmol CO mol air) and AMF on sucrose and phenylpropanoid metabolism, including coumarins, the most important bioactive metabolite in Ammi majus. eCO and AMF were applied, and different parameters have been assessed in A. majus such as changes in mycorrhizal colonization, plant biomass production, photosynthesis, and levels of N, P, and Ca besides the key metabolites and enzymes in sucrose and coumarins metabolic pathways. The present outcomes revealed that eCO and AMF individually or combined enhanced the plant biomass and photosynthesis as well as nutrient concentrations. Furthermore, the levels of sucrose, soluble sugars, glucose, fructose, and the activities of some key enzymes in their metabolism besides phenylpropanoids metabolites in shoot and root of A. majus have been enhanced by eCO and AMF especially when combined. Moreover, upregulation of sucrose is linked to phenylpropanoids metabolic pathway via upregulation of phenylalanine ammonia-lyase activity suggesting high coumarin biosynthesis. Generally, the synergistic effect of both treatments was noted for most of the investigated parameters compared to the individual effect. It could be concluded that the combined application of eCO and AMF affects A. majus global metabolism and induces accumulation of phyto-molecules, coumarin, which might improve its medicinal and pharmacological applications.
高水平的 CO（eCO）和丛枝菌根真菌（AMF）已被证明是促进植物生长和发育以及提高品质的成功环保剂。本研究旨在研究 eCO（620 μmol CO mol air）和 AMF 对蔗糖和苯丙烷代谢的影响，包括香豆素，这是 Ammi majus 中最重要的生物活性代谢物。应用了 eCO 和 AMF，并评估了 A. majus 中的不同参数，如菌根定植、植物生物量生产、光合作用以及氮、磷和钙等养分浓度的变化。此外，还评估了蔗糖和香豆素代谢途径中关键代谢物和酶的水平。研究结果表明，eCO 和 AMF 单独或联合增强了植物生物量和光合作用以及养分浓度。此外，eCO 和 AMF 还提高了 shoot 和 root 中蔗糖、可溶性糖、葡萄糖、果糖以及代谢过程中的一些关键酶的活性，以及苯丙烷代谢物的水平，尤其是当两者联合使用时。此外，蔗糖的上调与苯丙烷代谢途径有关，通过苯丙氨酸解氨酶活性的上调表明香豆素生物合成水平较高。总的来说，与单独处理相比，两种处理的协同作用在大多数研究参数中都得到了体现。可以得出结论，eCO 和 AMF 的联合应用会影响 A. majus 的整体代谢，并诱导植物分子如香豆素的积累，从而可能改善其药用和药理学应用。
