Caowen Sun, Run Xu, Xuchen Guo, Xulan Shang, Shengzuo Fang
National Key Laboratory for the Development and Utilization of Forest Food Resources, Nanjing Forestry University, Nanjing, 210037, China.
Co-Innovation Center for Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing, 210037, Jiangsu, China.
Sci Rep. 2025 Aug 5;15(1):28495. doi: 10.1038/s41598-025-12934-3.
Cyclocarya paliurus, endemic to the subtropical regions of southern China, holds significant potential as a health product, with its leaf-derived secondary metabolites commanding considerable value. This study aimed to investigate the secondary metabolites associated with graft success in C. paliurus by selecting three clones and three provenances as grafting scions and rootstocks. The survival rates of various graft combinations were meticulously recorded. Targeted metabolic profiling of the graft union was employed to analyze the flavonoid and phenylpropanoid biosynthesis pathways in both compatible and incompatible graft combinations. The results indicate that phenylpropanoids preferentially accumulate in compatible graft unions, while flavonoids are more abundant in incompatible unions. In the grafted seedlings of C. paliurus, ferulic acid, glycitein, dihydromyricetin, pinocembrin, and apigenin exhibited distinct accumulation patterns. These findings revealed the regulatory patterns within the phenylpropanoid biosynthesis pathway for predicting graft success in C. paliurus. The insights are valuable for the optimization of C. paliurus grafting practices and for future breeding programs aimed at enhancing the species' horticultural and economic potential.
青钱柳原产于中国南方亚热带地区，作为一种保健品具有巨大潜力，其叶片衍生的次生代谢产物具有相当高的价值。本研究旨在通过选择三个无性系和三个种源作为嫁接接穗和砧木，研究与青钱柳嫁接成功相关的次生代谢产物。精心记录了各种嫁接组合的成活率。采用靶向代谢谱分析嫁接愈合部位，分析亲和与不亲和嫁接组合中黄酮类和苯丙烷类生物合成途径。结果表明，苯丙烷类化合物优先在亲和嫁接愈合部位积累，而黄酮类化合物在不亲和愈合部位更为丰富。在青钱柳嫁接苗中，阿魏酸、黄豆黄素、二氢杨梅素、松属素和芹菜素表现出不同的积累模式。这些发现揭示了苯丙烷类生物合成途径中的调控模式，可用于预测青钱柳嫁接成功与否。这些见解对于优化青钱柳嫁接实践以及未来旨在提高该物种园艺和经济潜力的育种计划具有重要价值。
