• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

将中东呼吸综合征（MERS）抗原包裹于含α-半乳糖神经酰胺的脂质体中，可在免疫功能正常和白细胞减少的小鼠中引发更强的效应和记忆免疫反应。

Encapsulation of MERS antigen into α-GalCer-bearing-liposomes elicits stronger effector and memory immune responses in immunocompetent and leukopenic mice.

作者信息

Khan Masood Alam, Khan Arif, Alzohairy Mohammad A, Alruwetei Abdulmohsen M, Alsahli Mohammed A, Allemailem Khaled S, Alrumaihi Faris, Almatroudi Ahmad, Alhatlani Bader Y, Rugaie Osamah Al, Malik Ajamaluddin

机构信息

Department of Basic Health Sciences, College of Applied Medical Sciences, Qassim University, Buraydah, Saudi Arabia.

Department of Medical Laboratories, College of Applied Medical Sciences, Qassim University, Buraydah, Saudi Arabia.

出版信息

J King Saud Univ Sci. 2022 Jul;34(5):102124. doi: 10.1016/j.jksus.2022.102124. Epub 2022 May 27.

DOI:10.1016/j.jksus.2022.102124
PMID:35663348
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9135648/
Abstract

OBJECTIVES

Here, we prepared a liposome-based vaccine formulation containing Middle East Respiratory Syndrome Coronavirus papain-like protease (MERS-CoV-PLpro).

METHODS

A persistent leukopenic condition was induced in mice by injecting cyclophosphamide (CYP) three days before each dose of immunization. Mice were immunized on days 0, 14 and 21 with α-GalCer-bearing MERS-CoV PLpro-encapsulated DPPC-liposomes (α-GalCer-MERS-PLpro-liposomes or MERS-CoV PLpo-encapsulated DPPC-liposomes (MERS-PLpro-liposomes), whereas the antigen emulsified in Alum (MERS-PLpro-Alum) was taken as a control. On day 26, the blood was taken from the immunized mice to analyze IgG titer, whereas the splenocytes were used to analyze the lymphocyte proliferation and the level of cytokines. In order to assess the memory immune response, mice were given a booster dose after 150 days of the last immunization.

RESULTS

The higher levels of MERS-CoV-PLpro-specific antibody titer, IgG2a and lymphocyte proliferation were noticed in mice immunized with α-GalCer-MERS-PLpro-liposomes. Besides, the splenocytes from mice immunized with α-GalCer-MERS-PLpro-liposomes produced larger amounts of IFN-γ as compared to the splenocytes from MERS-PLpro-liposomes or MERS- PLpro-Alum immunized mice. Importantly, an efficient antigen-specific memory immune response was observed in α-GalCer-MERS-PLpro-liposomes immunized mice.

CONCLUSIONS

These findings suggest that α-GalCer-MERS-PLpro-liposomes may substantiate to be a successful vaccine formulation against MERS-CoV infection, particularly in immunocompromised individuals.

摘要

目的

在此，我们制备了一种基于脂质体的疫苗制剂，其包含中东呼吸综合征冠状病毒木瓜样蛋白酶（MERS-CoV-PLpro）。

方法

在每次免疫前三天，通过注射环磷酰胺（CYP）在小鼠中诱导持续性白细胞减少症。在第0、14和21天，用负载α-半乳糖神经酰胺的包裹MERS-CoV PLpro的二棕榈酰磷脂酰胆碱脂质体（α-GalCer-MERS-PLpro-脂质体）或包裹MERS-CoV PLpo的二棕榈酰磷脂酰胆碱脂质体（MERS-PLpro-脂质体）对小鼠进行免疫，而以明矾乳化的抗原（MERS-PLpro-明矾）作为对照。在第26天，从免疫的小鼠中采集血液以分析IgG滴度，而脾细胞用于分析淋巴细胞增殖和细胞因子水平。为了评估记忆免疫反应，在最后一次免疫150天后给小鼠加强剂量。

结果

在用α-GalCer-MERS-PLpro-脂质体免疫的小鼠中，观察到更高水平的MERS-CoV-PLpro特异性抗体滴度、IgG2a和淋巴细胞增殖。此外，与用MERS-PLpro-脂质体或MERS-PLpro-明矾免疫的小鼠的脾细胞相比，用α-GalCer-MERS-PLpro-脂质体免疫的小鼠的脾细胞产生了更多的干扰素-γ。重要的是，在α-GalCer-MERS-PLpro-脂质体免疫的小鼠中观察到了有效的抗原特异性记忆免疫反应。

结论

这些发现表明，α-GalCer-MERS-PLpro-脂质体可能被证实是一种针对MERS-CoV感染的成功疫苗制剂，特别是在免疫功能低下的个体中。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f77/9135648/db397e46a41a/gr7_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f77/9135648/be1400e257d8/gr1_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f77/9135648/5b61d9148713/gr2_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f77/9135648/859c214fc8dd/gr3_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f77/9135648/df8df63b387f/gr4_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f77/9135648/17e0017ac3b6/gr5_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f77/9135648/5ec493e7daf5/gr6_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f77/9135648/db397e46a41a/gr7_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f77/9135648/be1400e257d8/gr1_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f77/9135648/5b61d9148713/gr2_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f77/9135648/859c214fc8dd/gr3_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f77/9135648/df8df63b387f/gr4_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f77/9135648/17e0017ac3b6/gr5_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f77/9135648/5ec493e7daf5/gr6_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f77/9135648/db397e46a41a/gr7_lrg.jpg

相似文献

1
Encapsulation of MERS antigen into α-GalCer-bearing-liposomes elicits stronger effector and memory immune responses in immunocompetent and leukopenic mice.将中东呼吸综合征（MERS）抗原包裹于含α-半乳糖神经酰胺的脂质体中，可在免疫功能正常和白细胞减少的小鼠中引发更强的效应和记忆免疫反应。
J King Saud Univ Sci. 2022 Jul;34(5):102124. doi: 10.1016/j.jksus.2022.102124. Epub 2022 May 27.
2
Delivery of MERS antigen encapsulated in α-GalCer-bearing liposomes elicits stronger antigen-specific immune responses.载有 MERS 抗原的 α-GalCer 脂质体的递呈可引发更强的抗原特异性免疫应答。
J Drug Target. 2022 Sep;30(8):884-893. doi: 10.1080/1061186X.2022.2066681. Epub 2022 Apr 22.
3
Liposome-Mediated Delivery of MERS Antigen Induces Potent Humoral and Cell-Mediated Immune Response in Mice.脂质体介导的中东呼吸综合征抗原传递可诱导小鼠产生强烈的体液和细胞免疫应答。
Molecules. 2022 Jan 9;27(2):403. doi: 10.3390/molecules27020403.
4
Evaluation of pH-sensitive fusogenic polymer-modified liposomes co-loaded with antigen and α-galactosylceramide as an anti-tumor vaccine.评估负载抗原和α-半乳糖神经酰胺的pH敏感融合聚合物修饰脂质体作为抗肿瘤疫苗的效果。
J Vet Med Sci. 2018 Feb 9;80(2):197-204. doi: 10.1292/jvms.17-0491. Epub 2017 Dec 28.
5
Catalytic function and substrate specificity of the papain-like protease domain of nsp3 from the Middle East respiratory syndrome coronavirus.中东呼吸综合征冠状病毒nsp3木瓜蛋白酶样蛋白酶结构域的催化功能及底物特异性
J Virol. 2014 Nov;88(21):12511-27. doi: 10.1128/JVI.01294-14. Epub 2014 Aug 20.
6
Proteolytic processing, deubiquitinase and interferon antagonist activities of Middle East respiratory syndrome coronavirus papain-like protease.中东呼吸综合征冠状病毒木瓜蛋白酶样蛋白酶的蛋白水解加工、去泛素化酶和干扰素拮抗活性。
J Gen Virol. 2014 Mar;95(Pt 3):614-626. doi: 10.1099/vir.0.059014-0. Epub 2013 Dec 20.
7
MERS-CoV papain-like protease has deISGylating and deubiquitinating activities.MERS-CoV 木瓜蛋白酶样蛋白酶具有去 ISGylating 和去泛素化活性。
Virology. 2014 Feb;450-451:64-70. doi: 10.1016/j.virol.2013.11.040. Epub 2013 Dec 22.
8
Inhibitor recognition specificity of MERS-CoV papain-like protease may differ from that of SARS-CoV.中东呼吸综合征冠状病毒木瓜样蛋白酶的抑制剂识别特异性可能与严重急性呼吸综合征冠状病毒不同。
ACS Chem Biol. 2015 Jun 19;10(6):1456-65. doi: 10.1021/cb500917m. Epub 2015 Mar 16.
9
Assessing activity and inhibition of Middle East respiratory syndrome coronavirus papain-like and 3C-like proteases using luciferase-based biosensors.利用基于荧光素酶的生物传感器评估中东呼吸综合征冠状病毒木瓜蛋白酶样和 3C 样蛋白酶的活性和抑制作用。
J Virol. 2013 Nov;87(21):11955-62. doi: 10.1128/JVI.02105-13. Epub 2013 Aug 28.
10
Identification of Doxorubicin as Repurposing Inhibitory Drug for MERS-CoV PLpro.鉴定多柔比星作为 MERS-CoV PLpro 的再利用抑制剂药物。
Molecules. 2022 Nov 4;27(21):7553. doi: 10.3390/molecules27217553.

引用本文的文献

1
Mucosal immunity in upper and lower respiratory tract to MERS-CoV.上、下呼吸道对中东呼吸综合征冠状病毒的黏膜免疫
Front Immunol. 2024 Aug 30;15:1358885. doi: 10.3389/fimmu.2024.1358885. eCollection 2024.
2
Nanovaccines against Viral Infectious Diseases.抗病毒性传染病的纳米疫苗
Pharmaceutics. 2022 Nov 22;14(12):2554. doi: 10.3390/pharmaceutics14122554.

本文引用的文献

1
Liposome-Mediated Delivery of MERS Antigen Induces Potent Humoral and Cell-Mediated Immune Response in Mice.脂质体介导的中东呼吸综合征抗原传递可诱导小鼠产生强烈的体液和细胞免疫应答。
Molecules. 2022 Jan 9;27(2):403. doi: 10.3390/molecules27020403.
2
Role of NKT Cells during Viral Infection and the Development of NKT Cell-Based Nanovaccines.NKT细胞在病毒感染过程中的作用及基于NKT细胞的纳米疫苗的研发
Vaccines (Basel). 2021 Aug 26;9(9):949. doi: 10.3390/vaccines9090949.
3
Liposomal Ellagic Acid Alleviates Cyclophosphamide-Induced Toxicity and Eliminates the Systemic Infection in Leukopenic Mice.
脂质体鞣花酸减轻环磷酰胺诱导的毒性并消除白细胞减少症小鼠的全身感染。
Pharmaceutics. 2021 Jun 15;13(6):882. doi: 10.3390/pharmaceutics13060882.
4
Liposomal Nanovaccine Containing α-Galactosylceramide and Ganglioside GM3 Stimulates Robust CD8 T Cell Responses via CD169 Macrophages and cDC1.含有α-半乳糖神经酰胺和神经节苷脂GM3的脂质体纳米疫苗通过CD169巨噬细胞和cDC1刺激强烈的CD8 T细胞反应。
Vaccines (Basel). 2021 Jan 16;9(1):56. doi: 10.3390/vaccines9010056.
5
SARS-CoV2 and immunosuppression: A double-edged sword.SARS-CoV2 与免疫抑制：一把双刃剑。
Transpl Infect Dis. 2020 Dec;22(6):e13404. doi: 10.1111/tid.13404. Epub 2020 Jul 17.
6
High-Dose Cyclophosphamide Administration Orchestrates Phenotypic and Functional Alterations of Immature Dendritic Cells and Regulates Th Cell Polarization.大剂量环磷酰胺给药可调控未成熟树突状细胞的表型和功能改变，并调节Th细胞极化。
Front Pharmacol. 2020 May 25;11:775. doi: 10.3389/fphar.2020.00775. eCollection 2020.
7
Nanoparticle-based vaccine development and evaluation against viral infections in pigs.基于纳米颗粒的疫苗开发与评价在猪病毒性传染病中的应用。
Vet Res. 2019 Nov 6;50(1):90. doi: 10.1186/s13567-019-0712-5.
8
Middle East respiratory syndrome coronavirus (MERS-CoV): Impact on Saudi Arabia, 2015.中东呼吸综合征冠状病毒（MERS-CoV）：2015年对沙特阿拉伯的影响
Saudi J Biol Sci. 2018 Nov;25(7):1402-1405. doi: 10.1016/j.sjbs.2016.09.020. Epub 2016 Oct 1.
9
Comparison of Perceived and Observed Hand Hygiene Compliance in Healthcare Workers in MERS-CoV Endemic Regions.中东呼吸综合征冠状病毒流行地区医护人员手部卫生合规情况的感知与观察比较
Healthcare (Basel). 2018 Oct 7;6(4):122. doi: 10.3390/healthcare6040122.
10
Seasonal influenza vaccine in immunocompromised persons.免疫功能低下人群的季节性流感疫苗。
Hum Vaccin Immunother. 2018 Jun 3;14(6):1311-1322. doi: 10.1080/21645515.2018.1445446. Epub 2018 Mar 21.