成年果蝇视网膜的全组织免疫荧光标记。

Whole-mount immunofluorescent labeling of the adult fly retina.

机构信息

Institute of Biotechnology, National Tsing Hua University, Hsinchu 30031, Taiwan.

Institute of Systems Neuroscience, National Tsing Hua University, Hsinchu 30031, Taiwan.

出版信息

STAR Protoc. 2022 May 27;3(2):101430. doi: 10.1016/j.xpro.2022.101430. eCollection 2022 Jun 17.

DOI:10.1016/j.xpro.2022.101430

PMID:35664260

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9157552/

Abstract

The adult compound eye is an ideal model for studying biological questions. However, light microscopy of this tissue requires cumbersome embedding and sectioning. Here, we document detailed whole-mount procedures for immunolabeling the adult retina, enabling high-quality studies of fluorescent-tagged targets with straightforward preparations. We describe the steps for visualizing the nuclear lamina, membrane-associated protein, and actin-rich rhabdomere, but this robust protocol can apply to other cellular structures and target proteins. For complete details on the use and execution of this protocol, please refer to Chang et al. (2021).

摘要

成年复眼是研究生物学问题的理想模型。然而，对这种组织进行光学显微镜检查需要繁琐的包埋和切片。在这里，我们记录了详细的成年视网膜免疫标记全组织程序，使具有简单准备的荧光标记靶标能够进行高质量的研究。我们描述了可视化核层、膜相关蛋白和富含肌动蛋白的纤毛小体的步骤，但这种强大的方案可适用于其他细胞结构和靶标蛋白。如需详细了解本方案的使用和执行情况，请参考 Chang 等人（2021 年）的文献。