大型健康脐带血捐献者中人类细小病毒B19感染率的调查。

Investigation of human parvovirus B19 prevalence in a large healthy umbilical cord blood donors.

作者信息

Ramezany Hooman, Kheirandish Maryam, Samiee Shahram, Khosravifar Mina, Hashemi Seyedeh Melika

机构信息

Department of Immunology, Research Center of Blood Transfusion, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran.

Non-Communicable Diseases Research Center, Endocrinology and Metabolism Population Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran.

出版信息

Iran J Microbiol. 2022 Feb;14(1):119-124. doi: 10.18502/ijm.v14i1.8813.

DOI:10.18502/ijm.v14i1.8813

PMID:35664722

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9085544/

Abstract

BACKGROUND AND OBJECTIVES

Umbilical cord blood (UCB) was used to source hematopoietic stem cells in the past. Despite the apparent advantages of UCB transplantation, virus reactivation poses a considerable danger in allogeneic hematopoietic stem cell transplantation (HSCT). Human Parvovirus B19 is regarded as a potential threat to UCB contamination. This study aimed to evaluate the prevalence of parvovirus B19 in cord blood donors by Semi-Nested PCR. This study is the first largescale report of the B19 DNA in cord blood donors in Iran.

MATERIALS AND METHODS

A total of 691 umbilical cord blood were collected under standard procedure. Then, DNA from buffy coat and plasma were extracted, and semi-nested PCR was performed for all samples.

RESULTS

Two out of 691 samples (0.29%) indicated viremia in plasma and buffy coat.

CONCLUSION

In this line, designing and validating a quantitative PCR assay for detection, quantification, and discrimination of Human B19 DNA genotypes of cord blood donors is necessary to enhance the safety of this source of stem cells.

摘要

背景与目的

过去脐带血（UCB）被用于获取造血干细胞。尽管脐带血移植有明显优势，但病毒再激活在异基因造血干细胞移植（HSCT）中构成相当大的危险。人细小病毒B19被视为脐带血污染的潜在威胁。本研究旨在通过半巢式PCR评估脐带血捐献者中细小病毒B19的流行情况。本研究是伊朗关于脐带血捐献者中B19 DNA的首份大规模报告。