Cassinotti P, Burtonboy G, Fopp M, Siegl G
Institute for Clinical Microbiology and Immunology, St. Gallen, Switzerland.
J Med Virol. 1997 Nov;53(3):229-32.
A nested polymerase chain reaction assay (nPCR) was used to investigate the potential of human parvovirus B19 DNA to persist in blood or bone marrow samples obtained either from blood donors or cadaveric bone donors or from patients presenting with clinical signs of parvovirus B19 infection. The presence of parvovirus B19 specific antibody in blood was tested by enzyme immunoassay (EIA). B19 virus genome was not detected in any blood sample of 115 blood donors, of whom 92 (80%) had anti-B19 IgG antibody only as an indication of past infection. In contrast; B19 virus DNA was detected in the bone marrow of 4 out of 45 bone donors. Each one of the serum samples available for 3 of these 4 individuals contained anti-B19 IgG antibody. Among 84 patients with clinical manifestations of parvovirus B19 infection, 17 (20%) had B19 virus DNA in bone marrow. Eight of the latter patients had anti-B19 IgG antibody in their blood but neither anti-B19 IgG nor B19 virus DNA. These data document the ability of parvovirus B19 DNA to persist in the bone marrow of asymptomatic individuals and patients with parvovirus B19 infection suspected on clinical grounds.
采用巢式聚合酶链反应检测法（nPCR），研究人细小病毒B19 DNA在血液或骨髓样本中持续存在的可能性，这些样本取自献血者、尸体骨捐赠者或有细小病毒B19感染临床症状的患者。采用酶免疫分析法（EIA）检测血液中细小病毒B19特异性抗体。在115名献血者的任何血液样本中均未检测到B19病毒基因组，其中92人（80%）仅具有抗B19 IgG抗体，表明曾有过感染。相比之下，在45名骨捐赠者的4人的骨髓中检测到B19病毒DNA。这4人中的3人可获得的每份血清样本均含有抗B19 IgG抗体。在84例有细小病毒B19感染临床表现的患者中，17例（20%）骨髓中有B19病毒DNA。后一组患者中有8人血液中有抗B19 IgG抗体，但既无抗B19 IgG也无B19病毒DNA。这些数据证明了细小病毒B19 DNA在无症状个体以及临床上怀疑有细小病毒B19感染的患者骨髓中持续存在的能力。
