Department of Chemistry and Biochemistry, College of Natural Sciences and Mathematics, The University of Toledo, Toledo, OH 43606, USA; School of Green Chemistry and Engineering, The University of Toledo, Toledo, OH 43606, USA; Dr. Nina McClelland Laboratory for Water Chemistry and Environmental Analysis, The University of Toledo, Toledo, OH 43606, USA.
Department of Chemistry and Biochemistry, College of Natural Sciences and Mathematics, The University of Toledo, Toledo, OH 43606, USA; School of Green Chemistry and Engineering, The University of Toledo, Toledo, OH 43606, USA; Dr. Nina McClelland Laboratory for Water Chemistry and Environmental Analysis, The University of Toledo, Toledo, OH 43606, USA.
J Chromatogr B Analyt Technol Biomed Life Sci. 2022 Jul 1;1203:123308. doi: 10.1016/j.jchromb.2022.123308. Epub 2022 May 25.
This work proposes a new method for biomonitoring studies focused on the screening and quantification of xenobiotics in blood-derived samples. The performance of a polydimethylsiloxane/divinylbenzene/polydimethylsiloxane (PDMS/DVB/PDMS) biocompatible extraction phase was investigated for extraction of pesticides and pharmaceuticals from plasma samples via direct immersion solid-phase microextraction (SPME) prior to gas chromatography-mass spectrometry. Under the optimum extraction settings, which included an attentive optimization of the fiber rinsing conditions, the microextraction device was able to endure 100 consecutive extractions from undiluted and diluted plasma with an overall reproducibility up to 28% for all the analytes tested, except chlorpyrifos-methyl. Optimized conditions were used to validate a quantitative method using matrix-matched calibration with isotopically labeled internal standard correction. Accuracy and precision values obtained for analysis of bovine plasma were within 96-132% and 0.05-5.82% respectively. LLOQs for all the analytes were at 1 µg L and LDR ranged within 1-100 µg L. The applicability of this method to plasma from different species (human, rat, rabbit) was also investigated. This work represents the first step toward broader use of the biocompatible PDMS/DVB/PDMS extraction phases for analysis of multiclass xenobiotics in plasma and other complex biofluids.
本工作提出了一种新的方法，用于专注于筛选和定量血液衍生样本中异源生物的生物监测研究。研究了一种聚二甲基硅氧烷/二乙烯基苯/聚二甲基硅氧烷（PDMS/DVB/PDMS）生物相容的萃取相，用于通过直接浸入式固相微萃取（SPME）从血浆样品中萃取农药和药物，然后进行气相色谱-质谱分析。在最佳萃取条件下，包括对纤维冲洗条件的仔细优化，微萃取装置能够承受 100 次连续从未稀释和稀释的血浆中进行萃取，所有测试的分析物的整体重现性高达 28%，除了甲基毒死蜱。优化条件用于使用基质匹配校准和同位素标记内标校正进行定量方法验证。牛血浆分析的准确度和精密度值分别在 96-132%和 0.05-5.82%之间。所有分析物的 LLOQ 均为 1 µg L，LDR 范围在 1-100 µg L 之间。还研究了该方法在不同物种（人、大鼠、兔）的血浆中的适用性。这项工作是朝着更广泛地使用生物相容的 PDMS/DVB/PDMS 萃取相分析血浆和其他复杂生物流体中的多类异源生物迈出的第一步。
