International Iberian Nanotechnology Laboratory (INL), Av. Mestre José Veiga s/n, 4715-330, Braga, Portugal.
Instituto de Engenharia de Sistemas e Computadores-Microsistemas e Nanotecnologias (INESC MN), R. Alves Redol 9, 1000-029, Lisboa, Portugal.
Curr Genet. 2022 Aug;68(3-4):393-406. doi: 10.1007/s00294-022-01242-2. Epub 2022 Jun 6.
This work describes the newly isolated Klebsiella pneumoniae phage vB_KpnS-Carvaje that presents unique features in relation to other phages reported to date. These findings provide new insights into the diversity and evolutionary pathways of Klebsiella phages. The genome characterization of the Carvaje phage revealed that its genome length is approximately 57 kb with 99 open reading frames (ORFs), 33 of which have assigned functions while 66 are unknown. This phage differs from other sequenced Klebsiella phages, showing the closest resemblance (up to 65.32%) with Salmonella phages belonging to the Nonanavirus and Sashavirus genera. Comparisons at the amino acid level and phylogeny analysis among homologous genomes indicate that the Klebsiella Carvaje phage forms a novel sister taxon within the node of the Nonanaviruses and Sashaviruses cluster. Due to the unique features of the Carvaje phage, we propose the constitution of a new genus within the Caudoviricetes class. Further studies include the exploitation of this phage and its identified proteins for the control of Klebsiella infections and as recognition molecules in diagnostic methods.
这项工作描述了新分离的肺炎克雷伯菌噬菌体 vB_KpnS-Carvaje，与迄今为止报道的其他噬菌体相比，它具有独特的特征。这些发现为研究克雷伯氏噬菌体的多样性和进化途径提供了新的视角。Carvaje 噬菌体的基因组特征表明，其基因组长度约为 57kb，包含 99 个开放阅读框（ORFs），其中 33 个具有已知功能，66 个未知。该噬菌体与其他已测序的肺炎克雷伯氏噬菌体不同，与属于 Nonanavirus 和 Sashavirus 属的沙门氏菌噬菌体最为相似（相似度高达 65.32%）。氨基酸水平的比较和同源基因组的系统发育分析表明，肺炎克雷伯氏 Carvaje 噬菌体在 Nonanaviruses 和 Sashaviruses 聚类的节点内形成了一个新的姐妹分类群。由于 Carvaje 噬菌体的独特特征，我们建议在 Caudoviricetes 类中设立一个新属。进一步的研究包括利用这种噬菌体及其鉴定的蛋白质来控制克雷伯氏菌感染，并作为诊断方法中的识别分子。
