Feng Jiao, Li Fei, Sun Li, Dong Lina, Gao Liting, Wang Han, Yan Liyong, Wu Changxin
Institute of Biomedical Sciences, The Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education of China, The Key Laboratory of Medical Molecular Cell Biology of Shanxi Province, Shanxi University, Taiyuan, China.
Center for Clinical Laboratory, The Affiliated Taian City Central Hospital of Qingdao University, Taian, China.
Front Microbiol. 2023 Jan 30;14:1081715. doi: 10.3389/fmicb.2023.1081715. eCollection 2023.
Carbapenem-resistant is one of the primary bacterial pathogens that pose a significant threat to global public health because of the lack of available therapeutic options. Phage therapy shows promise as a potential alternative to current antimicrobial chemotherapies. In this study, we isolated a new phage vB_KpnS_SXFY507 against KPC-producing from hospital sewage. It had a short latent period of 20 min and a large burst size of 246 phages/cell. The host range of phage vB_KpnS_SXFY507 was relatively broad. It has a wide range of pH tolerance and high thermal stability. The genome of phage vB_KpnS_SXFY507 was 53,122 bp in length with a G + C content of 49.1%. A total of 81 open-reading frames (ORFs) and no virulence or antibiotic resistance related genes were involved in the phage vB_KpnS_SXFY507 genome. Phage vB_KpnS_SXFY507 showed significant antibacterial activity . The survival rate of larvae inoculated with SXFY507 was 20%. The survival rate of -infected larvae was increased from 20 to 60% within 72 h upon treatment with phage vB_KpnS_SXFY507. In conclusion, these findings indicate that phage vB_KpnS_SXFY507 has the potential to be used as an antimicrobial agent for the control of .
耐碳青霉烯类细菌是对全球公共卫生构成重大威胁的主要细菌病原体之一，因为缺乏有效的治疗选择。噬菌体疗法有望成为当前抗菌化疗的潜在替代方法。在本研究中，我们从医院污水中分离出一种针对产KPC细菌的新型噬菌体vB_KpnS_SXFY507。它的潜伏期短，为20分钟，裂解量高，为每个细胞246个噬菌体。噬菌体vB_KpnS_SXFY507的宿主范围相对较广。它具有广泛的pH耐受性和高热稳定性。噬菌体vB_KpnS_SXFY507的基因组长度为53,122 bp，G+C含量为49.1%。噬菌体vB_KpnS_SXFY507基因组中共包含81个开放阅读框（ORF），且没有毒力或抗生素抗性相关基因。噬菌体vB_KpnS_SXFY507显示出显著的抗菌活性。接种SXFY507的幼虫存活率为20%。在用噬菌体vB_KpnS_SXFY507处理后，感染[细菌名称未明确]的幼虫在72小时内存活率从20%提高到了60%。总之，这些发现表明噬菌体vB_KpnS_SXFY507有潜力用作控制[细菌名称未明确]的抗菌剂。
