Departamento de Medicina Veterinária, Universidade Vila Velha - UVV, Vila Velha, ES, Brazil.
Departamento de Veterinária, Universidade Federal de Viçosa - UFV, Viçosa, MG, Brazil.
Trop Anim Health Prod. 2022 Jun 6;54(4):198. doi: 10.1007/s11250-022-03197-5.
Cattle parasitic otitis caused by the nematode Rhabditis spp. is a serious health problem in Brazil, a situation which is confounded by lack of effective control measures. In vitro studies associating biological and chemical control as an alternative method showed promising results. The objective was to evaluate the combined use of Duddingtonia flagrans (AC001), 10% dimethylsulfoxide (DMSO), and 1.9% ivermectin for the in vivo control of Rhabditis spp., in naturally infected Gyr cattle. For this purpose, 48 animals, whose infection in both ears was diagnosed, were randomly assigned to 6 groups: group 1 (ivermectin 1.9%); group 2 (10% DMSO); group 3 (AC001); group 4 (ivermectin 1.9% + 10% DMSO w/v); group 5 (1.9% ivermectin + AC001 w/v); group 6 (10% DMSO + AC001 v/v). The treatments were performed in a single dose, in the right ears, with the left ears remaining untreated, as a control group. There was a significant reduction (p < 0.01) in the number of nematodes in the treated groups in relation to the control, with the following best efficacies: groups 1 and 2, 47% and 52.9%, respectively, 7 days after treatment; groups 3, 4, and 5, 47.8%, 48.6% and 36.7%, respectively, 14 days post-treatment; group 6, 38.4%, 21 days post-treatment. It was concluded that the combination of chemical compounds and D. flagrans in a single application was effective for the in vivo control of Rhabditis spp. in naturally infected cattle.
由线虫 Rhabditis spp. 引起的牛寄生虫性耳炎是巴西的一个严重健康问题，由于缺乏有效的控制措施，情况更加复杂。将生物和化学控制相结合作为替代方法的体外研究显示出了有希望的结果。本研究的目的是评估联合使用 Duddingtonia flagrans (AC001)、10%二甲亚砜 (DMSO) 和 1.9%伊维菌素对自然感染 Gyr 牛体内 Rhabditis spp. 的控制效果。为此，将 48 头双侧均被感染的动物随机分为 6 组：第 1 组（伊维菌素 1.9%）；第 2 组（10% DMSO）；第 3 组（AC001）；第 4 组（伊维菌素 1.9%+10% DMSO w/v）；第 5 组（伊维菌素 1.9%+AC001 w/v）；第 6 组（10% DMSO+AC001 v/v）。所有治疗均为单剂量右耳给药，左耳作为对照组不做任何处理。与对照组相比，所有处理组的线虫数量均显著减少（p<0.01），具有以下最佳疗效：第 1 组和第 2 组分别为 47%和 52.9%，在治疗后 7 天；第 3 组、第 4 组和第 5 组分别为 47.8%、48.6%和 36.7%，在治疗后 14 天；第 6 组为 38.4%，在治疗后 21 天。综上所述，在自然感染牛中，单一应用化学化合物和 D. flagrans 混合物对 Rhabditis spp. 的体内控制是有效的。
