Kämpfer I, Nissler K
Biomed Biochim Acta. 1986;45(10):1259-65.
The 113mIn ion is tightly bound to hemoglobin; less than 20% of the total amount of 113mIn present in the red cells are attached to the stroma. The extraction of the heme from purified hemoglobin with HCl/acetone mixture showed 99% of the 113mIn activity in the heme fraction. In comparison to the 51Cr and 99mTc isotopes known to be bound to the beta-chain of globin only, 20 and 30%, respectively, of their activities were found in the heme fraction. Acetyl acetone is necessary for effective labelling of erythrocytes with 113mIn. Only 7% of the acetyl acetone applied were found in the cells associated with the heme. It is not involved in the binding of 113mIn to hemoglobin, but facilitates the transport of the 113mIn ions into the cells.
113mIn离子与血红蛋白紧密结合；红细胞中存在的113mIn总量中，不到20%附着于基质。用HCl/丙酮混合物从纯化的血红蛋白中提取血红素，结果显示血红素部分中99%的113mIn活性。与已知仅与球蛋白β链结合的51Cr和99mTc同位素相比，它们分别有20%和30%的活性存在于血红素部分。乙酰丙酮是用113mIn有效标记红细胞所必需的。所应用的乙酰丙酮中只有7%存在于与血红素相关的细胞中。它不参与113mIn与血红蛋白的结合，但促进113mIn离子进入细胞。
