Siegenthaler G, Saurat J H
Biochem Biophys Res Commun. 1987 Mar 13;143(2):418-23. doi: 10.1016/0006-291x(87)91370-2.
Polyacrylamide gel electrophoresis and isoelectrofocusing followed by immunoblotting technique with an anti-human retinol-binding protein (RBP) serum were used to study holo-RBP and apo-RBP in human plasma. Three observations were made the technique allowed for the first time to directly and quantitatively analyse holo- and apo-RBP. Holo-RBP represented 97.86 +/- 0.78% and apo-RBP 1.94 +/- 0.73% of the total RBP. All-trans-retinoic acid (RA) was found to bind to apo-RBP and to significantly modify the tertiary structure of the protein; this raises the question of RBP involvement in the transport of RA. reconstitution of holo-RBP using apo-RBP from delipidized serum was achieved only after its incubation with natural all-trans-retinoids such as retinol, 3-dehydroretinol and retinoic acid but not with synthetic analogs of retinoic acid (13-cis-retinoic acid, TMMP, 13-cis-TMMP, TTNPB). It appears that RBP has a structure specificity for natural retinoids.
采用聚丙烯酰胺凝胶电泳和等电聚焦，随后用抗人视黄醇结合蛋白（RBP）血清进行免疫印迹技术，研究人血浆中的全RBP和脱辅基RBP。得出了三个观察结果：该技术首次能够直接定量分析全RBP和脱辅基RBP。全RBP占总RBP的97.86±0.78%，脱辅基RBP占1.94±0.73%。发现全反式维甲酸（RA）与脱辅基RBP结合并显著改变蛋白质的三级结构；这就提出了RBP是否参与RA转运的问题。仅在脱脂血清中的脱辅基RBP与天然全反式类视黄醇如视黄醇、3 - 脱氢视黄醇和维甲酸孵育后，而不是与维甲酸的合成类似物（13 - 顺式维甲酸、TMMP、13 - 顺式TMMP、TTNPB）孵育后，才能实现全RBP的重构。看来RBP对天然类视黄醇具有结构特异性。
