重印本：羟胺自动氧化过程中超氧自由基的生成及其超氧化物歧化酶的测定。
Reprint of: Generation of Superoxide Radical during Autoxidation of Hydroxylamine and an Assay for Superoxide Dismutase.
机构信息
Department of Bacteriology, Tottori University, School of Medicine, Yonago, Tottori, 683, Japan.
出版信息
Arch Biochem Biophys. 2022 Sep 15;726:109247. doi: 10.1016/j.abb.2022.109247. Epub 2022 Jun 4.
Accompanying the autoxidation of hydroxylamine at pH 10.2, nitroblue tetrazolium was reduced and nitrite was produced in the presence of EDTA. The rate of autoxidation was negligible below pH 8.0, but sharply increased with increasing pH. The reduction of nitroblue tetrazolium was inhibited by superoxide dismutase, indicating the participation of superoxide anion radical in the autoxidation. Hydrogen peroxide stimulated the autoxidation and superoxide dismutase inhibited the hydrogen peroxide-induced oxidation, results which suggest the participation of hydrogen peroxide in autoxidation and in the generation of superoxide radical. An assay for superoxide dismutase using autoxidation of hydroxylamine is described.
在 pH 值为 10.2 时，伴随羟胺的自动氧化，在 EDTA 的存在下，亚硝基四唑蓝被还原生成亚硝酸盐。在 pH 值低于 8.0 时，自动氧化的速率可以忽略不计，但随着 pH 值的增加而急剧增加。超氧化物歧化酶抑制了亚硝基四唑蓝的还原，表明超氧阴离子自由基参与了自动氧化。过氧化氢刺激自动氧化，超氧化物歧化酶抑制过氧化氢诱导的氧化，这表明过氧化氢参与了自动氧化和超氧自由基的生成。描述了一种使用羟胺自动氧化测定超氧化物歧化酶的方法。
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