一种简单的黄素蛋白——葡萄糖氧化酶产生超氧自由基的证据。
Evidence for superoxide radical production by a simple flavoprotein: glucose oxidase.
作者信息
al-Bekairi A M, Nagi M N, Shoeb H A, al-Sawaf H A
机构信息
Department of Pharmacology, College of Pharmacy, King Saud University, Riyadh, Kingdom of Saudi Arabia.
出版信息
Biochem Mol Biol Int. 1994 Sep;34(2):233-8.
Nitroblue tetrazolium was reduced to blue formazan during the oxidation of glucose by glucose oxidase. The rate of blue color formation was dependent on the concentrations of glucose, nitroblue tetrazolium and glucose oxidase. The rate of the reaction was negligible below pH 8.4, but sharply increased with increasing pH. The reduction of nitroblue tetrazolium was inhibited by superoxide dismutase, consistent with the participation of superoxide anion radical in the reaction.
在葡萄糖氧化酶氧化葡萄糖的过程中，硝基蓝四唑被还原为蓝色甲臜。蓝色形成的速率取决于葡萄糖、硝基蓝四唑和葡萄糖氧化酶的浓度。在pH 8.4以下，反应速率可忽略不计，但随着pH值升高而急剧增加。超氧化物歧化酶抑制硝基蓝四唑的还原，这与超氧阴离子自由基参与该反应一致。
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