Laboratoire Reproduction et Développement des Plantes, Université de Lyon, ENS de Lyon, UCB Lyon 1, CNRS, INRAE, F-69342 Lyon, France.
Trends Plant Sci. 2022 Oct;27(10):1049-1062. doi: 10.1016/j.tplants.2022.05.006. Epub 2022 Jun 3.
The plant cytoskeleton regulates fundamental biological processes, including cell division. How to experimentally perturb the cytoskeleton is a key question if one wants to understand the role of both actin filaments (AFs) and microtubules (MTs) in a given biological process. While a myriad of mutants are available, knock-out in cytoskeleton regulators, when nonlethal, often produce little or no phenotypic perturbation because such regulators are often part of a large family, leading to functional redundancy. In this review, alternative techniques to modify the plant cytoskeleton during plant cell division are outlined. The different pharmacological and genetic approaches already developed in cell culture, transient assays, or in whole organisms are presented. Perspectives on the use of optogenetics to perturb the plant cytoskeleton are also discussed.
植物细胞骨架调节基本的生物过程，包括细胞分裂。如果人们想要了解肌动蛋白丝 (AFs) 和微管 (MTs) 在特定生物过程中的作用，那么如何在实验中干扰细胞骨架是一个关键问题。虽然有大量的突变体可用，但在非致命的情况下，细胞骨架调节剂的敲除通常不会产生或少产生表型干扰，因为这些调节剂通常是一个大家族的一部分，导致功能冗余。在这篇综述中，概述了在植物细胞分裂过程中修饰植物细胞骨架的替代技术。介绍了已经在细胞培养、瞬时测定或整个生物体中开发的不同药理学和遗传学方法。还讨论了利用光遗传学来干扰植物细胞骨架的前景。
