Department of Pediatrics, University of Michigan Medical School, 1150 West Medical Center Drive, Ann Arbor, MI 48109, United States.
Department of Pediatrics, Case Western Reserve University School of Medicine, Cleveland, OH, United States.
J Cyst Fibros. 2022 Sep;21(5):766-768. doi: 10.1016/j.jcf.2022.05.011. Epub 2022 Jun 4.
Chronic polymicrobial airway infections are a hallmark of cystic fibrosis (CF) lung disease. Antibiotic therapy is a primary treatment of CF pulmonary exacerbations (PEx); however, the impact of episodic antibiotic treatment on airway bacterial communities has not been well described. We analyzed sputum samples from adults with CF obtained immediately before and during antibiotic treatment of PEx. Sequencing of the V4 region of the bacterial 16S ribosomal RNA gene was used to assess changes in bacterial community structure during antibiotic treatment. The peak impact of antibiotic treatment was observed by day four or five of treatment. These findings advance our understanding of bacterial community dynamics during antibiotic treatment of PEx and complement recent and ongoing studies evaluating the optimal duration of antibiotic therapy for PEx.
慢性多微生物气道感染是囊性纤维化 (CF) 肺部疾病的一个标志。抗生素治疗是 CF 肺部感染加重 (PEx) 的主要治疗方法；然而，间歇性抗生素治疗对气道细菌群落的影响尚未得到很好的描述。我们分析了 CF 成人在 PEx 抗生素治疗前和治疗期间立即获得的痰样本。使用细菌 16S 核糖体 RNA 基因 V4 区的测序来评估抗生素治疗期间细菌群落结构的变化。在治疗的第四天或第五天观察到抗生素治疗的峰值影响。这些发现增进了我们对 PEx 抗生素治疗期间细菌群落动态的理解，并补充了最近和正在进行的评估 PEx 抗生素治疗最佳持续时间的研究。
