大鼠肝脏微粒体单加氧酶系统对氨曲南反应性的性别差异。

Sex difference in responsiveness to Aztreonam of monooxygenase system in liver microsomes from rats.

作者信息

Horie T, Kitada M, Tanabe Y, Kanakubo Y

出版信息

Biochem Pharmacol. 1987 Apr 1;36(7):1053-7. doi: 10.1016/0006-2952(87)90413-8.

Abstract

Effect of successive administration of Aztreonam on microsomal monooxygenase system was investigated in male and female Sprague-Dawley rats. The activities of benzphetamine N-demethylase, aminopyrine N-demethylase, p-nitroanisole O-demethylase and aniline hydroxylase in liver microsomes from male rats were decreased dose-dependently by Aztreonam. On the contrary, the activities in liver microsomes from female rats were slightly increased rather than decreased by the administration of Aztreonam. In addition, Aztreonam was found to decrease the specific content of microsomal cytochrome P-450 in male rats but not in female rats. The decreases in the activities observed in male rats were accompanied by a parallel decrease in the specific content of cytochrome P-450. Furthermore, the results of quantitation of P-450 (M-1), one of the male specific forms of cytochrome P-450, indicated that the administration of Aztreonam resulted in a dose-dependent decrease in the content of P-450 (M-1) in liver microsomes from male rats.

摘要

在雄性和雌性斯普拉格-道利大鼠中研究了连续给予氨曲南对微粒体单加氧酶系统的影响。氨曲南使雄性大鼠肝微粒体中苄非他明N-脱甲基酶、氨基比林N-脱甲基酶、对硝基苯甲醚O-脱甲基酶和苯胺羟化酶的活性呈剂量依赖性降低。相反，给予氨曲南后，雌性大鼠肝微粒体中的活性略有增加而非降低。此外，发现氨曲南可降低雄性大鼠微粒体细胞色素P-450的比含量，但对雌性大鼠无此作用。在雄性大鼠中观察到的活性降低伴随着细胞色素P-450比含量的平行降低。此外，对细胞色素P-450的雄性特异性形式之一P-450（M-1）的定量结果表明，给予氨曲南导致雄性大鼠肝微粒体中P-450（M-1）的含量呈剂量依赖性降低。