Saget Sarah, Kappeler Laurent, Grandjean Valérie, Leneuve Patricia, Berthaut Isabelle, Faure Céline, Czernichow Sébastien, Racine Chrystèle, Lévy Rachel, Dupont Charlotte
Sorbonne Université, INSERM, Centre de Recherche St-Antoine, CRSA, 75012, Paris, France.
IHU-ICAN Institute of Cardiometabolism and Nutrition, Paris, France.
Basic Clin Androl. 2022 Jun 7;32(1):9. doi: 10.1186/s12610-022-00159-7.
Excess weight and metabolic disorders have a negative impact on male reproductive functions. The mechanisms involved are numerous and complex and epigenetic mechanisms may also be involved, notably through the small non-coding RNAs. Among them, microRNAs (miRNAs) are of particular interest. This preliminary study aimed to identify the miRNAs differentially enriched in seminal plasma related to metabolic disorders and if some are also associated with spermatic parameters alterations. One hundred and sixty men between 18 to 45 years, partners of infertile couple, were included in this cohort. The miRNAs associated with metabolism were selected from the literature and assayed by quantitative real-time PCR using TaqMan gene expression assays. A subset of those with an interesting profile in seminal plasma were secondarily tested in blood.
Among the 11 selected miRNAs, seven were detected in seminal plasma (miR10b, miR19a, miR19b, miR34b, miR34c, miR133b, miRlet7c). A negative correlation was observed between seminal miR19a levels and metabolic syndrome, blood glucose and C-peptide. Seminal miR19b levels were also negatively correlated with metabolic syndrome. Seminal miR34c levels were negatively correlated with body mass index (BMI) and waist circumference. Seminal miR133b levels were positively correlated with BMI, waist circumference and leptin levels. Interestingly, modifications of miRNAs in seminal plasma seem specific since highlighted above correlations were not retrieved in the blood plasma for the miR19a, 19b, 10b, 34c.
Few metabolic and anthropometric disorders are correlated with the level of specific miRNAs in seminal plasma. Further studies will be required to decipher if other small non-coding RNAs may also be correlated with metabolic and anthropometric disorders and to assess their potential implication in the alteration of reproductive functions in men with obesity or metabolic disorders.
Metabolic Syndrome and Male Infertility (Metasperme): Trial registration: NCT01974947 . Registered 18 July 2013.
超重和代谢紊乱对男性生殖功能有负面影响。涉及的机制众多且复杂，表观遗传机制也可能参与其中，尤其是通过小非编码RNA。其中，微小RNA（miRNA）特别受关注。这项初步研究旨在鉴定与代谢紊乱相关的精浆中差异富集的miRNA，以及是否有一些也与精子参数改变有关。160名年龄在18至45岁之间的男性，作为不孕夫妇的配偶，被纳入该队列。从文献中选择与代谢相关的miRNA，并使用TaqMan基因表达分析通过定量实时PCR进行检测。精浆中具有有趣特征的一部分样本随后在血液中进行检测。
在所选的11种miRNA中，有7种在精浆中被检测到（miR10b、miR19a、miR19b、miR34b、miR34c、miR133b、miRlet7c）。观察到精浆中miR19a水平与代谢综合征、血糖和C肽呈负相关。精浆中miR19b水平也与代谢综合征呈负相关。精浆中miR34c水平与体重指数（BMI）和腰围呈负相关。精浆中miR133b水平与BMI、腰围和瘦素水平呈正相关。有趣的是，精浆中miRNA的变化似乎具有特异性，因为上述miR19a、19b、10b、34c在血浆中未发现类似的相关性。
少数代谢和人体测量紊乱与精浆中特定miRNA的水平相关。需要进一步研究来解读其他小非编码RNA是否也与代谢和人体测量紊乱相关，并评估它们在肥胖或代谢紊乱男性生殖功能改变中的潜在影响。
代谢综合征与男性不育（Metasperme）：试验注册：NCT01974947。2013年7月18日注册。
