BidSi6 和 BidEL 同工型可作为预测结直肠腺瘤性息肉的潜在标志物。

BidSi6 and BidEL isoforms as a potential marker for predicting colorectal adenomatous polyps.

机构信息

Department of Genetics, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, P.O. Box: 193951495, Tehran, Iran.

Department of Cancer, Gastroenterology and Liver Disease Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

出版信息

BMC Med Genomics. 2022 Jun 6;15(1):129. doi: 10.1186/s12920-022-01282-0.

DOI:10.1186/s12920-022-01282-0

PMID:35668495

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9172139/

Abstract

BACKGROUND

As a well-known protein, Bid links the extrinsic and intrinsic apoptotic pathways and plays important roles in cell proliferation. In this study, we evaluated the expression of two isoforms of the Bid gene (BidSi6 and BidEL) in colorectal adenomatous polyps as a biomarker and investigated the relationship between their expression levels with clinicopathological factors.

METHODS

The expression of BidSi6 and BidEL isoforms in 22 pairs of Adenomatous polyps and adjust non-polyp tissues was measured by qReal-Time PCR and compared with 10 normal colon tissues. ROC curve was performed to examine the diagnostic capacity. Also, sequencing was performed for molecular identification of BidSi6 isoform in adenomatous polyp.

RESULTS

Our results showed that BidSi6 and BidEL isoforms were significantly overexpressed in Adenomatous polyps and non-polyp adjacent tissues from the same patients compared to that in normal colon tissues, but there was no significant expression between polyps and adjust non-polyp tissues. There were no significant correlations between the expression of two isoforms and other features of clinicopathology. The area under the curve of BidSi6 and BidEL isoforms indicated powerful diagnostic capability. The phylogenetic tree was constructed based on the sequence of idSi6 isoform, and the results showed that adenomatous polyp tissue and adjust non-polyp tissue were separated from healthy colorectal tissue and reference sequence (EU678292).

CONCLUSIONS

These findings suggest that BidSi6 and BidEL isoforms can be used as new potential biomarkers in adenomatous polyps.

摘要

背景

Bid 作为一种已知的蛋白，连接着外在和内在的凋亡途径，并在细胞增殖中发挥着重要作用。在本研究中，我们评估了两种 Bid 基因异构体（BidSi6 和 BidEL）在结直肠腺瘤性息肉中的表达情况，作为一种生物标志物，并研究了它们的表达水平与临床病理因素之间的关系。

方法

通过 qReal-Time PCR 测量了 22 对腺瘤性息肉及其对应的非息肉组织中 BidSi6 和 BidEL 异构体的表达情况，并与 10 例正常结肠组织进行了比较。绘制 ROC 曲线以检查诊断能力。还对腺瘤性息肉中的 BidSi6 异构体进行了测序，以进行分子鉴定。

结果

我们的结果表明，与正常结肠组织相比，BidSi6 和 BidEL 异构体在腺瘤性息肉和同一患者的非息肉相邻组织中均显著过表达，但息肉和对应的非息肉组织之间没有显著的表达差异。两种异构体的表达与其他临床病理特征之间没有显著相关性。BidSi6 和 BidEL 异构体的曲线下面积表明其具有强大的诊断能力。根据 idSi6 异构体的序列构建了系统发育树，结果表明，腺瘤性息肉组织和对应的非息肉组织与健康结直肠组织和参考序列（EU678292）分离。