Fritton H P, Igo-Kemenes T, Nowock J, Strech-Jurk U, Theisen M, Sippel A E
Biol Chem Hoppe Seyler. 1987 Feb;368(2):111-9. doi: 10.1515/bchm3.1987.368.1.111.
The pattern of DNase I-hypersensitive sites in the chromatin domain of the lysozyme gene was investigated in several organs and cell-types of the chicken. In the cluster of hypersensitive chromatin sites framing the gene, different classes of sites could be discerned: A subset was common to essentially all cells examined except for erythrocytes. Thus several highly nuclease susceptible structures exist around the gene even in its repressed state. Beside the promoter region a second site 6.1 kb upstream of the transcriptional start site of the gene strictly correlates with the transcriptionally active or potentially active state of the gene in both, oviduct cells and macrophages. A final class of sites is specific for the particular lysozyme expressing tissue and the presence of its members distinguish whether the gene is steroid regulated or in a steroid independent active mode.
在鸡的多个器官和细胞类型中，研究了溶菌酶基因染色质结构域中脱氧核糖核酸酶I超敏位点的模式。在构成该基因框架的超敏染色质位点簇中，可以识别出不同类型的位点：除红细胞外，基本上所有检测的细胞都有一个共同的子集。因此，即使在该基因的抑制状态下，其周围也存在几个高度易被核酸酶作用的结构。除启动子区域外，该基因转录起始位点上游6.1 kb处的第二个位点，在输卵管细胞和巨噬细胞中，都与该基因的转录活性或潜在活性状态严格相关。最后一类位点是特定于特定表达溶菌酶的组织的，其成员的存在区分该基因是受类固醇调节还是处于类固醇非依赖性活性模式。
