McConachie D R, O'Day D H
Biochem Cell Biol. 1986 Dec;64(12):1281-7. doi: 10.1139/o86-168.
In mated cultures (NC4 X V12) of Dictyostelium discoideum containing 1.0 mM CaCl2, cell fusion generates large numbers of binucleate cells which develop into zygote giant cells. In the absence of Ca2+, binucleate formation does not occur. When 1.0 mM CaCl2 is added to Ca2+-deficient cultures at 18 h, 50% of the cells fuse within 45 min producing large multinucleate syncytia. Small, presumptive gametes appear in Ca2+-deficient cultures and reach a peak of about 20% of the cell population by 10 h, but they maintain this plateau and do not fuse. Upon the addition of CaCl2, the presumptive gametes immediately fuse, producing binucleate cells which develop rapidly into morphologically distinct giant cells. Cell fusion continues, resulting in the formation of extremely large (40-80 microns diameter) multinucleate syncytia by 45 min. The induction of this extensive, synchronous cell fusion does not occur in the presence of other chloride salts and EGTA inhibits it, revealing that Ca+ is the regulatory ion.
在含有1.0 mM氯化钙的盘基网柄菌配对培养物（NC4×V12）中，细胞融合会产生大量双核细胞，这些双核细胞会发育成合子巨细胞。在没有钙离子的情况下，不会形成双核细胞。当在18小时时向缺乏钙离子的培养物中添加1.0 mM氯化钙时，50%的细胞会在45分钟内融合，产生大型多核合胞体。在缺乏钙离子的培养物中会出现小型的推测性配子，到10小时时达到细胞群体的约20%的峰值，但它们维持在这个水平且不融合。加入氯化钙后，推测性配子立即融合，产生双核细胞，这些双核细胞会迅速发育成形态上不同的巨细胞。细胞融合持续进行，到45分钟时会形成极其大（直径40 - 80微米）的多核合胞体。在存在其他氯化物盐的情况下不会诱导这种广泛的同步细胞融合，并且乙二醇双四乙酸会抑制它，这表明钙离子是调节离子。
