Graduate School of Engineering, Chiba University, 1-33 Yayoi, Inage, Chiba, 263-8522, Japan; Institute for Advanced Academic Research, Chiba University, 1-33 Yayoi, Inage, Chiba, 263-8522, Japan.
Department of Mechanical Engineering, Division of Fundamental Engineering, Graduate School of Science and Engineering, Chiba University, 1-33 Yayoi, Inage, Chiba, 263-8522, Japan.
Biosens Bioelectron. 2022 Sep 15;212:114432. doi: 10.1016/j.bios.2022.114432. Epub 2022 May 30.
A non-invasive imaging called as PCB-EIT imaging has been proposed in order to image spatio-temporal ion concentration distribution around cell spheroids in 0.1 s of temporal resolution by a newly developed electrical impedance tomographic sensor printed on circuit board (PCB-EIT sensor). To realize the high temporal resolution in PCB-EIT imaging, the temporal compensation by the ion transport impedance model interpolating the extracted resistance of extracellular solution R obtained from the Cole-Cole equation is employed. To confirm the performance of PCB-EIT imaging, the ion concentration distribution around three cell spheroid types (GFP type (GFPT), Histone type (HT) and wild type (WT)) which are electrically different due to green fluorescent protein expressed in cytoplasm and nucleus of MRC-5. As a result, spatio-temporal ion concentration distributions due to ion transport from cell spheroid are successfully reconstructed by PCB-EIT imaging. The images by PCB-EIT imaging are validated with those by fluorescence ratio imaging within the distribution error ε of 0.046 ± 0.0038 in maximum. For an evaluation of the ion diffusivity D of each cell spheroid type by the mass transfer simulation based on Fick's law, D of GFPT shows the highest value among the three cell types in the earlier time range from 4 s, while D of HT shows the highest one in the time range from 15 s, which indicates that PCB-EIT imaging is able to evaluate the ion transport characteristics of each cell type.
一种名为 PCB-EIT 成像的无创成像技术已经被提出，以便通过新开发的印刷电路板（PCB-EIT 传感器）上的电阻抗断层成像传感器以 0.1 秒的时间分辨率对细胞球体周围的时空离子浓度分布进行成像。为了在 PCB-EIT 成像中实现高时间分辨率，采用了通过离子传输阻抗模型对从科尔-科尔方程中提取的细胞外液电阻 R 进行插值的时间补偿。为了确认 PCB-EIT 成像的性能，研究了三种细胞球体类型（GFP 型（GFPT）、组蛋白型（HT）和野生型（WT））的离子浓度分布，由于细胞质和细胞核中表达的绿色荧光蛋白，这些细胞球体在电学上是不同的。MRC-5。结果，通过 PCB-EIT 成像成功重建了来自细胞球体的离子传输引起的时空离子浓度分布。通过 PCB-EIT 成像获得的图像与荧光比率成像的图像在最大分布误差 ε 为 0.046±0.0038 的范围内进行了验证。为了通过基于菲克定律的传质模拟对每种细胞球体类型的离子扩散系数 D 进行评估，GFPT 的 D 在 4 秒以内的早期时间范围内在三种细胞类型中表现出最高值，而 HT 的 D 在 15 秒的时间范围内表现出最高值，这表明 PCB-EIT 成像能够评估每种细胞类型的离子传输特性。
