Kawashima Daisuke, Li Songshi, Obara Hiromichi, Takei Masahiro
IEEE Trans Biomed Eng. 2021 Mar;68(3):1015-1023. doi: 10.1109/TBME.2020.3002980. Epub 2021 Feb 18.
Low-frequency impedance-based (LFI) cell discrimination as a novel non-destructive and non-invasive cell discrimination is proposed. LFI cell discrimination discriminates the cell type by considering an ion transport model in cell suspension. Ion transport model in cell suspension is constructed on the basis of Fick's laws of diffusion in the extracellular region under ion permeability P which represents the characteristics of cell type. P is achieved using the ion transport model equation through an iterative curve fitting to an ion concentration in extracellular region obtained from low-frequency impedance which is assumed to be linearly related to the ion concentration in extracellular region. In experiment, the electrical impedance spectra from the frequency of 200 kHz to 2.0 MHz are measured over time during producing ions from intracellular region to extracellular one in cell suspension using an impedance analyzer and an interdigitated array electrode system. As a target cell type, two different cell types based on Medical Research Council 5 (MRC-5), which are different in intracellular component are used. The curve fitting is performed for the low-frequency impedance at 200 kHz at which impedance reflects the ion concentration in extracellular region in order to obtain P of each cell type. As a result, each cell type has its own P. The proposed LFI cell discrimination successfully discriminates the cell type.
提出了基于低频阻抗（LFI）的细胞鉴别方法，这是一种新型的非破坏性、非侵入性细胞鉴别方法。LFI细胞鉴别通过考虑细胞悬液中的离子传输模型来区分细胞类型。细胞悬液中的离子传输模型是基于菲克扩散定律在离子渗透率P下的细胞外区域构建的，P代表细胞类型的特征。通过对从低频阻抗获得的细胞外区域离子浓度进行迭代曲线拟合来求解离子传输模型方程，从而得到P，假设低频阻抗与细胞外区域离子浓度呈线性关系。在实验中，使用阻抗分析仪和叉指阵列电极系统，在细胞悬液中从细胞内区域向细胞外区域产生离子的过程中，随时间测量200kHz至2.0MHz频率范围内的电阻抗谱。作为目标细胞类型，使用了基于医学研究委员会5（MRC-5）的两种不同细胞类型，它们在细胞内成分上有所不同。对200kHz处的低频阻抗进行曲线拟合，该频率下的阻抗反映细胞外区域的离子浓度，以获得每种细胞类型的P。结果，每种细胞类型都有其自身的P。所提出的LFI细胞鉴别方法成功地鉴别了细胞类型。
