Semina O V, Semenets T N, Kurilets E S, Man'ko V M, Poverennyĭ A M
Biull Eksp Biol Med. 1987 Apr;103(4):444-6.
CFU-S differentiation and regeneration kinetics in the spleen and femur was studied after treatment of bone marrow cells with RAMB serum. The effect of thymocytes on the rate of CFU-S regeneration was also investigated. It was found that CFU-S regeneration in the spleen was similar in RAMBS-treated and intact cell populations on days 4-14 after transplantation. On the contrary, the rate of CFU-S regeneration in the femur was slower in RAMBS-treated than in intact bone marrow cells. However, the growth rate in the femur could be restored to the normal level by the administration of freshly isolated syngeneic thymocytes to mice pre-injected with RAMBS-treated CFU-S population. The treatment of bone marrow suspension with RAMB serum did not affect the differentiation of spleen colonies. It is suggested that RAMBS eliminates cell population regulating CFU-S proliferation, without affecting its differentiation.
在用RAMB血清处理骨髓细胞后，研究了脾脏和股骨中CFU-S的分化和再生动力学。还研究了胸腺细胞对CFU-S再生速率的影响。发现在移植后第4 - 14天，经RAMBS处理的细胞群体和完整细胞群体中脾脏CFU-S的再生情况相似。相反，经RAMBS处理的股骨中CFU-S的再生速率比完整骨髓细胞中的慢。然而，通过给预先注射经RAMBS处理的CFU-S群体的小鼠注射新鲜分离的同基因胸腺细胞，股骨中的生长速率可恢复到正常水平。用RAMB血清处理骨髓悬液不影响脾集落的分化。提示RAMBS消除了调节CFU-S增殖的细胞群体，而不影响其分化。
