An immunologic comparison between bone marrow and spleen-derived pluripotent hemopoietic stem cells (CFU-S) of mouse : effect of rabbit anti-mouse brain serum.

We used rabbit anti-mouse brain serum (RAMBS), with known activity against pluripotent hemopoietic stem cells (CFU-S) of mice, to compare the proportions of CFU-S from bone marrow and spleen which express the brain-associated CFU-S antigen(s). Eighty percent of marrow and 77% of spleen-derived CFU-S were inactivated by RAMBS. This suggests that these two populations of CFU-S are antigenically similar with respect to the brain-associated CFU-S antigen(s); this is in contrast to functional differences which are known to exist between the two populations of CFU-S.