对四氧嘧啶诱导的糖尿病大鼠具有抗氧化应激、肝毒性和高血糖的保护作用。
protects against oxidative stress, hepatotoxicity and hyperglycaemia in alloxan-induced diabetic rats.
作者信息
Orumwense Godwin Ezemwenghian, Osagie Aishat Mary, Omage Sylvia Oghogho, Omage Kingsley, Azeke Marshall Arebojie
机构信息
Department of Biochemistry, Faculty of Life Sciences, Ambrose Alli University, Ekpoma, Edo State Nigeria.
Department of Human Nutrition, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, UK.
出版信息
J Diabetes Metab Disord. 2022 Mar 18;21(1):669-680. doi: 10.1007/s40200-022-01029-9. eCollection 2022 Jun.
BACKGROUND
is commonly used in traditional medical practices for the management of diseases like diabetes and its complications. This study seeks to establish a scientific rationale for this practice.
METHODS
Thirty Wistar rats were randomly and equally grouped into six. Alloxan was used to induce diabetes in the rats in groups 2 to 6. The diabetic rats in group 2 were treated with glibenclamide, while those in group 3 were not treated. Also, the diabetic rats in groups 4, 5 and 6 were, respectively, treated with the ethanol extracts of the stem, root and leaf of . After 28 days of treatment, blood and organ samples were collected for biochemical studies.
RESULTS
possesses high amounts of useful phytochemicals. It also exhibits high total reducing capacity, FRAP activity, DPPH and ABTS scavenging ability. The inhibition of the α-glucosidase and α-amylase activities by the methanol extracts of stem, leaf and root were significantly ( < 0.05) higher than that of glibenclamide. Administration of extracts to the alloxan-induced diabetic rats caused significant ( < 0.05) decreases in the blood glucose, total bilirubin, AST, ALT, and ALP of the treated groups as compared to that of the untreated group. Treatment with the extracts also resulted in significantly ( < 0.05) lower LPO and significantly ( < 0.05) higher levels of GSH, SOD and CAT.
CONCLUSION
extracts exhibited antioxidative, hepatoprotective and hypoglycaemic properties which are similar to that of the standard drug, glibenclamide.
背景
在传统医学实践中常用于治疗糖尿病及其并发症等疾病。本研究旨在为这种实践建立科学依据。
方法
将30只Wistar大鼠随机均分为6组。用四氧嘧啶诱导第2至6组大鼠患糖尿病。第2组的糖尿病大鼠用格列本脲治疗，而第3组的大鼠不治疗。此外，第4、5和6组的糖尿病大鼠分别用[植物名称]的茎、根和叶的乙醇提取物治疗。治疗28天后，采集血液和器官样本进行生化研究。
结果
[植物名称]含有大量有用的植物化学物质。它还具有较高的总还原能力、FRAP活性、DPPH和ABTS清除能力。[植物名称]茎、叶和根的甲醇提取物对α-葡萄糖苷酶和α-淀粉酶活性的抑制作用显著（P<0.05）高于格列本脲。与未治疗组相比，给四氧嘧啶诱导的糖尿病大鼠施用[植物名称]提取物导致治疗组的血糖、总胆红素、AST、ALT和ALP显著（P<0.05）降低。用提取物治疗还导致LPO显著（P<0.05）降低，GSH、SOD和CAT水平显著（P<0.05）升高。
结论
[植物名称]提取物表现出与标准药物格列本脲相似的抗氧化、保肝和降血糖特性。
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