Efosa John Osarenren, Omage Kingsley, Azeke Marshall Arebojie
Department of Physical Laboratory Technology, School of Applied Sciences and Technology, Auchi Polytechnic, Edo State, Nigeria.
Department of Biochemistry, College of Basic Medical Sciences, Igbinedion University Okada, Edo State, Nigeria.
Toxicol Rep. 2023 Sep 6;11:177-188. doi: 10.1016/j.toxrep.2023.09.005. eCollection 2023 Dec.
The effects of different drying temperatures on the hypolipidemic, antioxidant, and antihypertensive potential of calyx was evaluated. The calyx were dried under different temperature conditions (- 58 °C, 30 °C, 40 °C, and 50 °C), and extracted with a solvent mixture of ethanol and water (1:4 % w/v). To induce hypertension, the rats were administered with 40 mg/kg body weight dose of N-nitro L-arginine methyl-ester (L-NAME), via the intra-gastric route. extract was administered orally, at varying doses (250, 500, and 1000 mg/kg) to the rats. Afterwards, the hypolipidemic, antioxidant, and antihypertensive potentials of the extracts were evaluated using standard validated methods. Induction with L-NAME significantly (p < 0.05) increased the total cholesterol, triglyceride, and LDL levels, significantly decreased the HDL levels; significantly (p < 0.05) increased the levels of LPO/MDA, HO, and decreased GPx, and SOD activities; significantly (p < 0.05) increased the pressures (diastolic and systolic); significantly (p < 0.05) increased ACE and arginase activities, glucose level, and significantly decreased nitric oxide activity. Treatment with extract significantly (p < 0.05) reversed these trends in the hypertensive experimental rats. The hypolipidemic, antioxidant, and antihypertensive properties of the extract from the calyx of , which varies with the drying temperatures of the calyx, portends its potential as a curative agent in the treatment of hypertensive conditions, and other cardiovascular diseases.
评估了不同干燥温度对花萼降血脂、抗氧化和降压潜力的影响。将花萼在不同温度条件下（-58℃、30℃、40℃和50℃）干燥，并用乙醇和水的混合溶剂（1:4，w/v）提取。为诱导高血压，通过胃内途径给大鼠施用40mg/kg体重剂量的N-硝基-L-精氨酸甲酯（L-NAME）。将提取物以不同剂量（250、500和1000mg/kg）口服给予大鼠。之后，使用标准验证方法评估提取物的降血脂、抗氧化和降压潜力。用L-NAME诱导显著（p<0.05）增加了总胆固醇、甘油三酯和低密度脂蛋白水平，显著降低了高密度脂蛋白水平；显著（p<0.05）增加了脂质过氧化/丙二醛、血红素加氧酶水平，并降低了谷胱甘肽过氧化物酶和超氧化物歧化酶活性；显著（p<0.05）增加了血压（舒张压和收缩压）；显著（p<0.05）增加了血管紧张素转换酶和精氨酸酶活性、血糖水平，并显著降低了一氧化氮活性。用提取物治疗显著（p<0.05）逆转了高血压实验大鼠中的这些趋势。花萼提取物的降血脂、抗氧化和降压特性随花萼干燥温度而变化，预示其在治疗高血压病症和其他心血管疾病方面作为治疗剂的潜力。
