Beijing National Laboratory for Molecular Sciences, CAS Key Laboratory of Analytical Chemistry for Living Biosystems, CAS Research/Education Center for Excellence in Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Zhongguancun North First Street 2, Beijing, 100190, People's Republic of China.
School of Chemistry, University of Chinese Academy of Sciences, Beijing, 100049, China.
Mikrochim Acta. 2022 Jun 8;189(7):246. doi: 10.1007/s00604-022-05308-3.
The development of hydrazone bond-oriented epitope imprinting strategy is reported to synthesize the polymeric binders for the selective recognition of a protein-β-microglobulin through either its N- or C-terminal epitope. The dynamic reversibility of hydrazone bond facilitated not only the oriented assembly of the template peptide hydrazides onto the substrate but also the efficient removal of them from the imprinted cavities. The well-defined surface imprinted layer was successfully constructed through the precise control over the polymerization of silicate esters. Binding performance of the C-terminal peptide imprinted nanocomposite was significantly improved after tuning the non-covalent interactions using the sequence-matching aromatic co-monomers. The dissociation constant (K) between the optimized nanocomposite and epitope peptide was 0.5 µmol L. The nanomaterial was utilized for the selective extraction and determination of β-microglobulin from human urine by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and HPLC-UV with satisfied recoveries of 93.1-112.3% in a concentration range 1.0-50.0 μg⋅mL.
报道了一种基于腙键的表位印迹策略的发展，用于合成聚合物结合剂，通过蛋白质-β-微球蛋白的 N 端或 C 端表位对其进行选择性识别。腙键的动态可逆性不仅促进了模板肽酰肼在基底上的定向组装，而且还促进了它们从印迹空穴中的有效去除。通过对硅酸盐酯聚合的精确控制，成功构建了具有明确表面印迹层的纳米复合材料。通过使用序列匹配的芳香族共聚单体来调整非共价相互作用，显著提高了 C 端肽印迹纳米复合材料的结合性能。优化后的纳米复合材料与表位肽之间的离解常数 (K) 为 0.5 μmol·L。该纳米材料通过基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF MS）和 HPLC-UV 用于选择性地从人尿中提取和测定β-微球蛋白，在 1.0-50.0 μg·mL 浓度范围内的回收率为 93.1-112.3%。
