Beijing National Laboratory for Molecular Sciences, CAS Key Laboratory of Analytical Chemistry for Living Biosystems, CAS Research/Education Center for Excellence in Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, China; School of Chemistry, University of Chinese Academy of Sciences, Beijing, 100049, China.
Beijing National Laboratory for Molecular Sciences, CAS Key Laboratory of Analytical Chemistry for Living Biosystems, CAS Research/Education Center for Excellence in Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, China; School of Chemistry, University of Chinese Academy of Sciences, Beijing, 100049, China.
Anal Chim Acta. 2021 Apr 15;1154:338301. doi: 10.1016/j.aca.2021.338301. Epub 2021 Feb 9.
As a kind of artificial recognition material, molecularly imprinted polymers (MIPs) offer a promising perspective to be developed as synthetic chemical binders capable of selectively recognize biomacromolecules. However, owing to the large size and conformational flexibility of proteins and peptides, imprinting of these biomacromolecules remains a challenge. Novel imprinting strategies still need exploration for the improvement of recognition performance of MIPs. Herein, we developed a hydrazone bond-oriented surface imprinting strategy for an endogenous peptide hormone, human atrial natriuretic peptide (ANP). Surface-oriented imprinting of peptide via reversible covalent bond anchoring approach increased the orientation homogeneity of imprinted cavities as well as the utility of templates. The prepared nanoparticles exhibited high selectivity and fast recognition kinetics for ANP epitope. The dissociation constant between ANP epitope and MIP was measured as 5.3 μM. The applicability of the material in real samples was verified by the selective magnetic extraction of ANP from human plasma samples. This hydrazone bond-oriented surface imprinting strategy provides an alternative approach for the separation of peptides or proteins in complex bio-samples.
作为一种人工识别材料，分子印迹聚合物（MIPs）为开发能够选择性识别生物大分子的合成化学结合物提供了很有前景的前景。然而，由于蛋白质和肽的体积大和构象灵活性，这些生物大分子的印迹仍然是一个挑战。为了提高 MIPs 的识别性能，仍需要探索新的印迹策略。在此，我们开发了一种腙键导向的表面印迹策略，用于内源性肽激素，人心房利钠肽（ANP）。通过可逆共价键锚定方法对肽进行表面印迹，增加了印迹腔的取向均一性和模板的利用率。所制备的纳米颗粒对 ANP 表位表现出高选择性和快速识别动力学。通过测量 ANP 表位和 MIP 之间的解离常数，确定为 5.3 μM。该材料在人血浆样品中的选择性磁性提取验证了其在实际样品中的适用性。这种腙键导向的表面印迹策略为复杂生物样品中的肽或蛋白质的分离提供了一种替代方法。
