Reproduction and Development Laboratory, CIISA - Centro de Investigação Interdisciplinar em Sanidade Animal, Faculdade de Medicina Veterinária, Universidade de Lisboa, Avenida da Universidade Técnica, Lisbon, Portugal.
Centro de Investigação Interdisciplinar Egas Moniz (CiiEM), Instituto Universitário Egas Moniz, Egas Moniz - Cooperativa de Ensino Superior, Crl, Quinta da Granja, Monte da Caparica, Portugal.
Methods Mol Biol. 2022;2472:173-185. doi: 10.1007/978-1-0716-2201-8_14.
The Notch signaling pathway is an important determinant of cell diversity and identity in most developing embryonic tissues. The pathway components are expressed dynamically, and their function is critical for embryonic survival.This protocol addresses the immunolocalization of Notch pathway components by confocal microscopy.
Notch 信号通路是大多数发育中胚胎组织中细胞多样性和特征的重要决定因素。该通路的组成部分呈动态表达，其功能对胚胎存活至关重要。本方案通过共聚焦显微镜技术来检测 Notch 通路组成部分的免疫定位。
