研究肾活检中 NOTCH 信号通路的激活。

Studying the NOTCH Signaling Pathway Activation in Kidney Biopsies.

机构信息

Cellular and Molecular Biology in Renal and Vascular Pathology Laboratory, Fundación Instituto de Investigación Sanitaria-Fundación Jiménez Díaz-Universidad Autónoma Madrid, Madrid, Spain.

Red de Investigación Renal (REDINREN), Instituto de Salud Carlos III, Madrid, Spain.

出版信息

Methods Mol Biol. 2022;2472:187-196. doi: 10.1007/978-1-0716-2201-8_15.

DOI:10.1007/978-1-0716-2201-8_15

PMID:35674901

Abstract

The NOTCH signaling pathway is an evolutionarily conserved family of transmembrane receptors, ligands, and transcription factors. The NOTCH signaling is activated in many biological processes including nephrogenesis, tubulogenesis, and glomerulogenesis, as well as during pathological situations. Activation of Notch signaling is characterized by successive proteolytic cleavages triggered by the interaction between membrane-bound Notch receptors and ligands expressed on neighboring cells. In chronic kidney diseases, activation of the canonical NOTCH signaling pathway has been described. The following protocols will allow the direct assessment of Jagged-1/NOTCH signaling activation in biopsies of patients with chronic kidney diseases and in murine experimental models of renal damage.

摘要

NOTCH 信号通路是一个进化上保守的跨膜受体、配体和转录因子家族。NOTCH 信号在许多生物学过程中被激活，包括肾发生、肾小管发生和肾小球发生，以及在病理情况下。Notch 信号的激活特征是由相邻细胞上表达的膜结合 Notch 受体与配体之间的相互作用触发的连续蛋白水解切割。在慢性肾脏病中，已经描述了经典 NOTCH 信号通路的激活。以下方案将允许直接评估慢性肾脏病患者活检标本和肾脏损伤的小鼠实验模型中 Jagged-1/NOTCH 信号的激活。