高压冷冻和透射电子显微镜观察耳念珠菌细胞壁的超微结构。

High-Pressure Freezing and Transmission Electron Microscopy to Visualize the Ultrastructure of the C. auris Cell Wall.

机构信息

Institute of Medical Sciences (IMS), School of Medicine, Medical Sciences and Nutrition, University of Aberdeen, Aberdeen, UK.

出版信息

Methods Mol Biol. 2022;2517:189-201. doi: 10.1007/978-1-0716-2417-3_15.

DOI:10.1007/978-1-0716-2417-3_15

PMID:35674955

Abstract

Transmission electron microscopy (TEM) is the main technique used to study the ultrastructure of biological samples. Chemical fixation was considered the main method for preserving samples for TEM; however, it is a relatively slow method of fixation and can result in morphological alterations. Cryofixation using high-pressure freezing (HPF) overcomes the limitations of chemical fixation by preserving samples instantly. Here, we describe our HPF methods optimized for visualizing Candida auris at the ultrastructural level.

摘要

透射电子显微镜（TEM）是用于研究生物样本超微结构的主要技术。化学固定被认为是用于 TEM 的主要保存样品的方法；然而，它是一种相对较慢的固定方法，并且可能导致形态改变。使用高压冷冻（HPF）的冷冻固定克服了化学固定的局限性，可立即保存样品。在这里，我们描述了优化用于在超微结构水平上观察耳念珠菌的 HPF 方法。