Using rapid freeze and freeze-substitution for the preparation of yeast cells for electron microscopy and three-dimensional analysis.
作者信息
Giddings T H, O'Toole E T, Morphew M, Mastronarde D N, McIntosh J R, Winey M
机构信息
Department of Molecular, Cellular and Developmental Biology, Porter Biosciences, University of Colorado-Boulder, Boulder, Colorado 80309, USA.
出版信息
Methods Cell Biol. 2001;67:27-42. doi: 10.1016/s0091-679x(01)67003-1.
Abstract
摘要
相似文献
1
Using rapid freeze and freeze-substitution for the preparation of yeast cells for electron microscopy and three-dimensional analysis.使用快速冷冻和冷冻置换法制备用于电子显微镜和三维分析的酵母细胞。
Methods Cell Biol. 2001;67:27-42. doi: 10.1016/s0091-679x(01)67003-1.
2
The transition of cells of the fission yeast beta-tubulin mutant nda3-311 as seen by freeze-substitution electron microscopy. Requirement of functional tubulin for spindle pole body duplication.通过冷冻置换电子显微镜观察裂殖酵母β-微管蛋白突变体nda3-311细胞的转变。功能性微管蛋白对纺锤体极体复制的需求。
J Cell Sci. 1990 Jun;96 ( Pt 2):275-82. doi: 10.1242/jcs.96.2.275.
3
Three-dimensional reconstruction and analysis of mitotic spindles from the yeast, Schizosaccharomyces pombe.粟酒裂殖酵母有丝分裂纺锤体的三维重建与分析
J Cell Biol. 1993 Jan;120(1):141-51. doi: 10.1083/jcb.120.1.141.
4
Mitosis in the fission yeast Schizosaccharomyces pombe as revealed by freeze-substitution electron microscopy.通过冷冻置换电子显微镜揭示的裂殖酵母粟酒裂殖酵母中的有丝分裂。
J Cell Sci. 1986 Feb;80:253-68. doi: 10.1242/jcs.80.1.253.
5
High-pressure freezing is a powerful tool for visualization of Schizosaccharomyces pombe cells: ultra-low temperature and low-voltage scanning electron microscopy and immunoelectron microscopy.高压冷冻是用于可视化粟酒裂殖酵母细胞的强大工具：超低温和低电压扫描电子显微镜及免疫电子显微镜。
J Electron Microsc (Tokyo). 2006 Apr;55(2):75-88. doi: 10.1093/jmicro/dfl014. Epub 2006 Jun 16.
6
In vitro approaches for the study of microtubule nucleation at the fission yeast spindle pole body.
Methods Cell Biol. 2001;67:167-77. doi: 10.1016/s0091-679x(01)67012-2.
7
Improved preservation of ultrastructure in difficult-to-fix organisms by high pressure freezing and freeze substitution: I. Drosophila melanogaster and Strongylocentrotus purpuratus embryos.通过高压冷冻和冷冻置换改善难固定生物体超微结构的保存：I. 黑腹果蝇和紫海胆胚胎
Microsc Res Tech. 1993 Apr 15;24(6):465-73. doi: 10.1002/jemt.1070240603.
8
Resinless section electron microscopy of HeLa cell mitotic architecture.HeLa细胞有丝分裂结构的无树脂切片电子显微镜观察
Proc Natl Acad Sci U S A. 1986 Dec;83(23):8996-9000. doi: 10.1073/pnas.83.23.8996.
9
High pressure freezing, electron microscopy, and immuno-electron microscopy of Tetrahymena thermophila basal bodies.嗜热四膜虫基体的高压冷冻、电子显微镜及免疫电子显微镜观察
Methods Mol Biol. 2009;586:227-41. doi: 10.1007/978-1-60761-376-3_12.
10
Preparing Fission Yeast for Electron Microscopy.为电子显微镜制备裂殖酵母。
Cold Spring Harb Protoc. 2017 Jan 3;2017(1):2017/1/pdb.prot091314. doi: 10.1101/pdb.prot091314.
引用本文的文献
1
Centromeres in the thermotolerant yeast K. marxianus mediate attachment to a single microtubule.耐热酵母马克斯克鲁维酵母中的着丝粒介导与单个微管的附着。
Chromosome Res. 2025 Jul 3;33(1):14. doi: 10.1007/s10577-025-09772-4.
2
Centromeres in the thermotolerant yeast K. marxianus mediate attachment to a single microtubule.耐热酵母马克斯克鲁维酵母中的着丝粒介导与单个微管的附着。
Res Sq. 2025 Apr 23:rs.3.rs-6173630. doi: 10.21203/rs.3.rs-6173630/v1.
3
Centromeres in the thermotolerant yeast mediate attachment to a single microtubule.耐热酵母中的着丝粒介导与单个微管的附着。
bioRxiv. 2025 Jan 27:2025.01.24.634737. doi: 10.1101/2025.01.24.634737.
4
Plasticity of the mitotic spindle in response to karyotype variation.有丝分裂纺锤体对核型变化的可塑性。
Curr Biol. 2024 Aug 5;34(15):3416-3428.e4. doi: 10.1016/j.cub.2024.06.058. Epub 2024 Jul 22.
5
High-Pressure Freezing and Transmission Electron Microscopy to Visualize the Ultrastructure of the C. auris Cell Wall.高压冷冻和透射电子显微镜观察耳念珠菌细胞壁的超微结构。
Methods Mol Biol. 2022;2517:189-201. doi: 10.1007/978-1-0716-2417-3_15.
6
Proteasome activity modulates amyloid toxicity.蛋白酶体活性调节淀粉样毒性。
FEMS Yeast Res. 2022 Mar 9;22(1). doi: 10.1093/femsyr/foac004.
7
A short perinuclear amphipathic α-helix in Apq12 promotes nuclear pore complex biogenesis.Apq12 中的一个短核周两性 α-螺旋促进核孔复合体的生物发生。
Open Biol. 2021 Nov;11(11):210250. doi: 10.1098/rsob.210250. Epub 2021 Nov 24.
8
Pcp1/pericentrin controls the SPB number in fission yeast meiosis and ploidy homeostasis.Pcp1/中心粒外周蛋白控制裂殖酵母减数分裂中的纺锤体极体数量和倍性稳态。
J Cell Biol. 2022 Jan 3;221(1). doi: 10.1083/jcb.202104099. Epub 2021 Nov 8.
9
Local chromatin fiber folding represses transcription and loop extrusion in quiescent cells.局部染色质纤维折叠抑制静止细胞中的转录和环挤出。
Elife. 2021 Nov 4;10:e72062. doi: 10.7554/eLife.72062.
10
A mutation in budding yeast affecting nuclear envelope insertion of the spindle pole body.一种影响芽殖酵母纺锤体极体核膜插入的突变。
MicroPubl Biol. 2021 Sep 16;2021. doi: 10.17912/micropub.biology.000463. eCollection 2021.
本文引用的文献
1
Mapping the three-dimensional organization of microtubules in mitotic spindles of yeast.绘制酵母有丝分裂纺锤体中微管的三维结构。
Trends Cell Biol. 1996 Jun;6(6):235-9. doi: 10.1016/0962-8924(96)40003-4.
2
Macromolecular differentiation of Golgi stacks in root tips of Arabidopsis and Nicotiana seedlings as visualized in high pressure frozen and freeze-substituted samples.在高压冷冻和冷冻置换样品中观察到的拟南芥和烟草幼苗根尖高尔基体堆叠的大分子分化。
Protoplasma. 1990;157(1-3):75-91. doi: 10.1007/BF01322640.
3
Altered dosage of the Saccharomyces cerevisiae spindle pole body duplication gene, NDC1, leads to aneuploidy and polyploidy.酿酒酵母纺锤体极体复制基因NDC1的剂量改变会导致非整倍体和多倍体。
Proc Natl Acad Sci U S A. 1999 Aug 31;96(18):10200-5. doi: 10.1073/pnas.96.18.10200.
4
High-voltage electron tomography of spindle pole bodies and early mitotic spindles in the yeast Saccharomyces cerevisiae.酿酒酵母纺锤极体和早期有丝分裂纺锤体的高压电子断层扫描
Mol Biol Cell. 1999 Jun;10(6):2017-31. doi: 10.1091/mbc.10.6.2017.
5
Localization of core spindle pole body (SPB) components during SPB duplication in Saccharomyces cerevisiae.酿酒酵母中纺锤体极体（SPB）核心组件在SPB复制过程中的定位。
J Cell Biol. 1999 May 17;145(4):809-23. doi: 10.1083/jcb.145.4.809.
6
High-pressure freezing for preservation of high resolution fine structure and antigenicity for immunolabeling.用于保存高分辨率精细结构和免疫标记抗原性的高压冷冻法。
Methods Mol Biol. 1999;117:77-97. doi: 10.1385/1-59259-201-5:77.
7
Three-dimensional transmission electron microscopy and its application to mitosis research.三维透射电子显微镜及其在有丝分裂研究中的应用。
Methods Cell Biol. 1999;61:81-111. doi: 10.1016/s0091-679x(08)61976-7.
8
Saccharomyces cerevisiae Ndc1p is a shared component of nuclear pore complexes and spindle pole bodies.酿酒酵母Ndc1p是核孔复合体和纺锤极体的共同组成部分。
J Cell Biol. 1998 Dec 28;143(7):1789-800. doi: 10.1083/jcb.143.7.1789.
9
cut11(+): A gene required for cell cycle-dependent spindle pole body anchoring in the nuclear envelope and bipolar spindle formation in Schizosaccharomyces pombe.cut11(+)：裂殖酵母中细胞周期依赖性纺锤体极体锚定在核膜以及双极纺锤体形成所必需的一个基因。
Mol Biol Cell. 1998 Oct;9(10):2839-55. doi: 10.1091/mbc.9.10.2839.
10
High-pressure freezing and freeze-substitution of native rat brain: suitability for preservation and immunoelectron microscopic localization of myelin glycolipids.大鼠原生脑的高压冷冻和冷冻置换：用于髓鞘糖脂的保存及免疫电子显微镜定位的适用性
J Neurosci Res. 1998 Aug 15;53(4):465-74. doi: 10.1002/(SICI)1097-4547(19980815)53:4<465::AID-JNR8>3.0.CO;2-4.
Zotero 插件