Department of Medicine, University of Wisconsin, Madison, WI, USA.
Department of Medical Microbiology and Immunology, University of Wisconsin, Madison, WI, USA.
Methods Mol Biol. 2022;2517:251-258. doi: 10.1007/978-1-0716-2417-3_20.
Candida auris spreads person to person in hospitals and other healthcare facilities. The heightened capacity for C. auris to colonize skin contributes to the difficulty in eradicating this drug-resistant and deadly pathogen in nosocomial settings. Models for the study of C. auris skin colonization are critical for understanding this virulence trait. In light of the similarities between the skin properties of humans and pigs, pigs represent an ideal model for the investigation of skin-C. auris interactions. Here, we describe how to utilize porcine skin for ex vivo studies of C. auris colonization.
耳念珠菌在医院和其他医疗机构中通过人与人之间传播。耳念珠菌定植在皮肤上的能力增强，导致在医院环境中消除这种耐药性和致命病原体变得更加困难。研究耳念珠菌皮肤定植的模型对于了解这种毒力特征至关重要。鉴于人类和猪的皮肤特性相似，猪是研究皮肤-耳念珠菌相互作用的理想模型。在这里，我们描述了如何利用猪皮进行耳念珠菌定植的离体研究。
