Department of Pharmacy, Faculty of Science, National University of Singapore, Block S9, Level 15, 4 Science Drive 2, Singapore 117544, Singapore.
NUS Graduate School for Integrative Sciences and Engineering, 21 Lower Kent Ridge Road, Singapore 119077, Singapore.
Biomacromolecules. 2022 Jul 11;23(7):2803-2813. doi: 10.1021/acs.biomac.2c00176. Epub 2022 Jun 8.
Tissue engineering involves the transplantation of stem cell-laden hydrogels as synthetic constructs to replace damaged tissues. However, their time-consuming fabrication procedures are hurdles to widespread application in clinics. Fortunately, similar to cell banking, synthetic tissues could be cryopreserved for subsequent central distribution. Here, we report the use of trehalose and gellan gum as biomacromolecules to form a cryopreservable yet directly implantable hydrogel system for adipose-derived stem cell (ADSC) delivery. Through a modified cell encapsulation method and a preincubation step, adequate cryoprotection was afforded at 0.75 M trehalose to the encapsulated ADSCs. At this concentration, trehalose demonstrated lower propensity to induce apoptosis than 10% DMSO, the current gold standard cryoprotectant. Moreover, when cultured along with trehalose after thawing, the encapsulated ADSCs retained their stem cell-like phenotype and osteogenic differentiation capacity. Taken together, this study demonstrates the feasibility of an "off-the-shelf" biomacromolecule-based synthetic tissue to be applied in widespread tissue engineering applications.
组织工程涉及将含有干细胞的水凝胶作为合成结构移植，以替代受损组织。然而，其耗时的制造程序是在临床上广泛应用的障碍。幸运的是，类似于细胞库，合成组织可以冷冻保存，以便随后进行集中分配。在这里，我们报告使用海藻糖和结冷胶作为生物大分子，形成可冷冻保存但可直接植入的水凝胶系统，用于脂肪来源干细胞（ADSC）的输送。通过改良的细胞包封方法和预孵育步骤，在 0.75 M 海藻糖的浓度下，包封的 ADSC 获得了足够的冷冻保护。在该浓度下，海藻糖诱导细胞凋亡的倾向低于当前的金标准冷冻保护剂 10%DMSO。此外，在解冻后与海藻糖一起培养时，包封的 ADSC 保留了其干细胞样表型和成骨分化能力。总之，这项研究证明了“现成的”基于生物大分子的合成组织在广泛的组织工程应用中的可行性。
