College of New Materials and New Energies, Shenzhen Technology University, Shenzhen, 518118, China.
School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, Wuhan, 430070, China.
Adv Sci (Weinh). 2022 Aug;9(23):e2202326. doi: 10.1002/advs.202202326. Epub 2022 Jun 8.
Early detection and diagnosis are vitally important in reducing the mortality rate of fatal diseases but require highly sensitive detection of biomarkers. Presently, detection methods with the highest sensitivity require in vitro processing, while in vivo compatible fluorescence detections require a much higher concentration of biomarkers or limit of detection (LOD). In this paper, a fundamentally new strategy for ultrasensitive detection based on color-switchable lasing with a cavity-enhanced reduction of LOD is demonstrated, down to 1.4 × 10 mg ml for a quantitative detection, lower than both the fluorescence method and plasmonic enhanced method. For a qualitative or a yes/no type of detection, the LOD is as low as 10 mg ml . The approach in this work is based on a dye-embedded, in vivo compatible, polystyrene-sphere cavity, penetrable by biomarkers. A polystyrene sphere serves the dual roles of a laser cavity and an in vivo bio-reactor, in which dye molecules react with a biomarker, reporting biomarker information through lasing signals. The cavity-enhanced emission and lasing with only a single biomarker molecule per cavity allow improved visual distinguishability via color changes. Furthermore, when combined with a narrow-band filter, the color-switchable lasers act as an "on-off" logic signal and can be integrated into multiplexing detection assay biochips.
早期发现和诊断对于降低致命疾病的死亡率至关重要，但需要高度敏感的生物标志物检测。目前，灵敏度最高的检测方法需要体外处理，而与体内兼容的荧光检测则需要更高浓度的生物标志物或检测限 (LOD)。本文提出了一种基于具有腔增强 LOD 降低的可切换激光的超灵敏检测的全新策略，定量检测的 LOD 低至 1.4×10-1.4×10mg/ml，低于荧光法和等离子体增强法。对于定性或是/否类型的检测，LOD 低至 10mg/ml。这项工作的方法基于可穿透生物标志物的染料嵌入的、与体内兼容的聚苯乙烯球腔。聚苯乙烯球同时充当激光腔和体内生物反应器的双重作用，其中染料分子与生物标志物反应，通过激光信号报告生物标志物信息。腔增强发射和每个腔仅有单个生物标志物分子的激光允许通过颜色变化提高视觉可区分性。此外，当与窄带滤波器结合时，可切换颜色的激光器充当“开/关”逻辑信号，并且可以集成到多重检测分析生物芯片中。
