转录组分析揭示了慢性阻塞性肺疾病（COPD）和牙周炎之间的病理生理关系。
Transcriptomic analysis reveals pathophysiological relationship between chronic obstructive pulmonary disease (COPD) and periodontitis.
机构信息
Department of Stomatology, Jiangxi Provincial People's Hospital, The First Affiliated Hospital of Nanchang Medical College, Aiguo Road No. 152, Nanchang, 330006, Jiangxi Province, China.
Department of General Practice, Jiangxi Provincial People's Hospital, The First Affiliated Hospital of Nanchang Medical College, Aiguo Road No. 152, Nanchang, 330006, Jiangxi Province, China.
出版信息
BMC Med Genomics. 2022 Jun 8;15(1):130. doi: 10.1186/s12920-022-01278-w.
BACKGROUND
The aim of this study was to detect potential crosstalk genes, pathways and immune cells between periodontitis and chronic obstructive pulmonary disease (COPD).
METHODS
Chronic periodontitis (CP, GSE156993) and COPD (GSE42057, GSE94916) datasets were downloaded. Differential expressed genes (DEGs; p < 0.05) were assessed and screened for overlapping results, following functional pathway enrichment analyses (p < 0.05). The xCell method was used to assess immune cell infiltration relationship between CP and COPD. Features of the detected cross-talk genes were revealed using conventional Recursive Feature Elimination (RFE) algorithm in R project. Receiver-operating characteristic curves were applied to evaluate the predictive value of the genes. Furthermore, Pearson correlation analysis was performed on crosstalk markers and infiltrating immune cells in CP and COPD, respectively.
RESULTS
A total of 904 DEGs of COPD and 763 DEGs of CP were acquired, showing 22 overlapping DEGs between the two diseases. Thereby 825 nodes and 923 edges were found in the related protein-protein-interaction network. Eight immune cell pairs were found to be highly correlated to both CP and COPD (|correlation coefficients |> 0.5 and p-value < 0.05). Most immune cells were differently expressed between COPD and CP. RFE identified three crosstalk genes, i.e. EPB41L4A-AS1, INSR and R3HDM1. In correlation analysis, INSR was positively correlated with Hepatocytes in CP (r = 0.6714, p = 0.01679) and COPD (r = 0.5209, p < 0.001). R3HDM was positively correlated with Th1 cells in CP (r = 0.6783, p = 0.0153) and COPD (r = 0.4120, p < 0.01).
CONCLUSION
EPB41L4A-AS1, INSR and R3HDM1 are potential crosstalk genes between COPD and periodontitis. R3HDM was positively correlated with Th1 cells in both diseases, while INSR was positively correlated with Hepatocytes in periodontitis and COPD, supporting a potential pathophysiological relationship between periodontitis and COPD.
背景
本研究旨在检测牙周炎和慢性阻塞性肺疾病（COPD）之间潜在的串扰基因、通路和免疫细胞。
方法
下载慢性牙周炎（CP，GSE156993）和 COPD（GSE42057，GSE94916）数据集。评估差异表达基因（DEGs;p<0.05），并进行功能通路富集分析（p<0.05）以筛选重叠结果。使用 xCell 方法评估 CP 和 COPD 之间免疫细胞浸润的关系。使用 R 项目中的常规递归特征消除（RFE）算法揭示检测到的串扰基因的特征。应用受试者工作特征曲线评估基因的预测价值。此外，还分别对 CP 和 COPD 中的串扰标志物和浸润免疫细胞进行 Pearson 相关性分析。
结果
共获得 COPD 的 904 个 DEGs 和 CP 的 763 个 DEGs，发现两种疾病之间有 22 个重叠的 DEGs。因此，在相关的蛋白质-蛋白质相互作用网络中发现了 825 个节点和 923 个边缘。发现 8 对免疫细胞对 CP 和 COPD 均具有高度相关性（|相关系数|>0.5，p 值<0.05）。大多数免疫细胞在 COPD 和 CP 之间的表达不同。RFE 鉴定出三个串扰基因，即 EPB41L4A-AS1、INSR 和 R3HDM1。在相关性分析中，INSR 与 CP 中的肝细胞呈正相关（r=0.6714，p=0.01679）和 COPD（r=0.5209，p<0.001）。R3HDM 与 CP 中的 Th1 细胞呈正相关（r=0.6783，p=0.0153）和 COPD（r=0.4120，p<0.01）。
结论
EPB41L4A-AS1、INSR 和 R3HDM1 是 COPD 和牙周炎之间的潜在串扰基因。R3HDM 与两种疾病中的 Th1 细胞呈正相关，而 INSR 与牙周炎和 COPD 中的肝细胞呈正相关，这支持了牙周炎和 COPD 之间潜在的病理生理学关系。
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