神经肽作为连接牙周炎和重度抑郁症的共享遗传串扰。
Neuropeptides as the Shared Genetic Crosstalks Linking Periodontitis and Major Depression Disorder.
机构信息
Department of Oral and Maxillofacial Surgery, Dental Hospital, The First Affiliated Hospital of Harbin Medical University, 23 Youzheng Road, Nangang District, Harbin, 150001 Heilongjiang, China.
Department of Prosthodontics, Dental Hospital, The First Affiliated Hospital of Harbin Medical University, 23 Youzheng Road, Nangang District, Harbin, 150001 Heilongjiang, China.
出版信息
Dis Markers. 2021 Oct 21;2021:3683189. doi: 10.1155/2021/3683189. eCollection 2021.
BACKGROUND
The aim of this study was at investigating the association between major depressive disorder (MDD) and periodontitis based on crosstalk genes and neuropeptides.
METHODS
Datasets for periodontitis (GSE10334, GSE16134, and GSE23586) and MDD (GSE38206 and GSE39653) were downloaded from GEO. Following batch correction, a differential expression analysis was applied (MDD: ∣log2FC | >0 and periodontitis ∣log2FC | ≥0.5, < 0.05). The neuropeptide data were downloaded from NeuroPep and NeuroPedia. Intersected genes were potential crosstalk genes. The correlation between neuropeptides and crosstalk genes in MDD and periodontitis was analyzed with Pearson correlation coefficient. Subsequently, regression analysis was performed to calculate the differentially regulated link. Cytoscape was used to map the pathways of crosstalk genes and neuropeptides and to construct the protein-protein interaction network. Lasso regression was applied to screen neuropeptides, whereby boxplots were created, and receiver operating curve (ROC) analysis was conducted.
RESULTS
The MDD dataset contained 30 case and 33 control samples, and the periodontitis dataset contained 430 case and 139 control samples. 35 crosstalk genes were obtained. A total of 102 neuropeptides were extracted from the database, which were not differentially expressed in MDD and periodontitis and had no intersection with crosstalk genes. Through lasso regression, 9 neuropeptides in MDD and 43 neuropeptides in periodontitis were obtained. Four intersected neuropeptide genes were obtained, i.e., ADM, IGF2, PDYN, and RETN. The results of ROC analysis showed that IGF2 was highly predictive in MDD and periodontitis. ADM was better than the other three genes in predicting MDD disease. A total of 13 crosstalk genes were differentially coexpressed with four neuropeptides, whereby FOSB was highly expressed in MDD and periodontitis.
CONCLUSION
The neuropeptide genes ADM, IGF2, PDYN, and RETN were intersected between periodontitis and MDD, and FOSB was a crosstalk gene related to these neuropeptides on the transcriptomic level. These results are a basis for future research in the field, needing further validation.
背景
本研究旨在基于共话基因和神经肽探讨重度抑郁症（MDD）和牙周炎之间的关联。
方法
从 GEO 下载牙周炎（GSE10334、GSE16134 和 GSE23586）和 MDD（GSE38206 和 GSE39653）数据集。进行批次校正后，进行差异表达分析（MDD：∣log2FC∣＞0 且牙周炎∣log2FC∣≥0.5，<0.05）。神经肽数据从 NeuroPep 和 NeuroPedia 下载。相交的基因是潜在的共话基因。用 Pearson 相关系数分析 MDD 和牙周炎中神经肽和共话基因之间的相关性。然后进行回归分析以计算差异调控关系。用 Cytoscape 映射共话基因和神经肽的通路并构建蛋白质-蛋白质相互作用网络。用 Lasso 回归筛选神经肽，绘制箱线图并进行接收器工作特征（ROC）分析。
结果
MDD 数据集包含 30 例病例和 33 例对照样本，牙周炎数据集包含 430 例病例和 139 例对照样本。得到 35 个共话基因。从数据库中提取了 102 种神经肽，它们在 MDD 和牙周炎中无差异表达且与共话基因无交集。通过 Lasso 回归，得到 MDD 中的 9 种神经肽和牙周炎中的 43 种神经肽。得到 4 个相交的神经肽基因，即 ADM、IGF2、PDYN 和 RETN。ROC 分析结果显示，IGF2 在 MDD 和牙周炎中具有高度预测性。ADM 在预测 MDD 疾病方面优于其他三个基因。共 13 个共话基因与 4 个神经肽差异共表达，其中 FOSB 在 MDD 和牙周炎中高表达。
结论
神经肽基因 ADM、IGF2、PDYN 和 RETN 在牙周炎和 MDD 之间存在交集，转录组水平上 FOSB 是与这些神经肽相关的共话基因。这些结果为该领域的进一步研究奠定了基础，有待进一步验证。
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