丝状肌动蛋白A调控姜黄素对膀胱癌细胞放射增敏作用的机制研究
Mechanism Study on Radiosensitization Effect of Curcumin in Bladder Cancer Cells Regulated by Filamin A.
作者信息
Wang Zhenfan, He Shuqing, Jiang Minjun, Li Xue, Chen Na
机构信息
Soochow University Affiliated Suzhou Ninth Hospital, Suzhou, China.
State Key Laboratory of Radiation Medicine and Protection, School of Radiation Medicine and Protection, Soochow University, Suzhou, China.
出版信息
Dose Response. 2022 Jun 2;20(2):15593258221100997. doi: 10.1177/15593258221100997. eCollection 2022 Apr-Jun.
OBJECTIVE
To study the radiosensitization effect of curcumin, a natural product with anti-inflammatory and anti-cancer properties, in bladder cancer cells and identify the specific role of FLNA gene in that process.
METHODS
CCK-8 method was initially adopted to identify the proper interventional concentration of curcumin. T24 bladder cancer cells were subjected to CCK-8, flow cytometry, and colony formation assay to study the cell biological behaviors under different interventions. γ-H2AX test was performed to test the level of damage in T24 cells. RT-qPCR and Western blot were conducted to measure FLNA mRNA and protein levels.
RESULTS
Low-dose curcumin (10, 20 μM) following X-ray exposure resulted in increased DNA damage, augmented apoptosis, and reduced proliferation of T24 cells. Certain radiosensitization was demonstrated when curcumin was applied at 10 μM. Additionally, elevation of FLNA gene and protein levels was also indicated upon combination treatment.
CONCLUSION
Low-dose curcumin has certain radiosensitization effect in bladder cancer, where FLNA plays a certain regulatory role.
目的
研究具有抗炎和抗癌特性的天然产物姜黄素对膀胱癌细胞的放射增敏作用，并确定FLNA基因在此过程中的具体作用。
方法
最初采用CCK-8法确定姜黄素的合适干预浓度。对T24膀胱癌细胞进行CCK-8、流式细胞术和集落形成试验，以研究不同干预下的细胞生物学行为。进行γ-H2AX试验以检测T24细胞中的损伤水平。进行RT-qPCR和蛋白质免疫印迹法以测量FLNA mRNA和蛋白质水平。
结果
X射线照射后低剂量姜黄素（10、20 μM）导致T24细胞DNA损伤增加、凋亡增加和增殖减少。当以10 μM应用姜黄素时显示出一定的放射增敏作用。此外，联合治疗后FLNA基因和蛋白质水平也升高。
结论
低剂量姜黄素在膀胱癌中具有一定的放射增敏作用，其中FLNA发挥一定的调节作用。
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