在新冠疫情期间，运用多重聚合酶链反应检测法对一所幼儿园中3型人副流感病毒感染暴发进行早期检测。
Use of a multiplex polymerase chain reaction assay for the early detection of an outbreak of human parainfluenza virus type 3 infection in a nursery school during the COVID-19 pandemic.
作者信息
Suzuki Jun, Endo Shiro, Mizuno Tomoki, Takahashi Shota, Horiuchi Yukiko, Ami Yuri, Imai Haruka, Shimada Daishi, Yoshida Makiko, Kaku Mitsuo, Seki Masafumi
机构信息
Division of Infectious Diseases, Tohoku Medical and Pharmaceutical University Hospital, Sendai City, Miyagi 983-8512, Japan.
Department of Infection Prevention and Control, Tohoku Medical and Pharmaceutical University Hospital, Sendai City, Miyagi 983-8512, Japan.
出版信息
Infect Prev Pract. 2022 May 25;4(3):100221. doi: 10.1016/j.infpip.2022.100221. eCollection 2022 Sep.
INTRODUCTION
Although outbreaks of parainfluenza virus type 3 (PIV-3) have been reported in children, to our knowledge none have been reported in a nursery school. As the symptoms of PIV-3 infection are similar to those of COVID-19 infection, accurate diagnosis of PIV-3 and other respiratory viruses is important during the COVID-19 pandemic.
AIMS
We experienced an outbreak of upper respiratory symptoms at a nursery school in Miyagi Prefecture, Japan, from 29/5/2021 to 13/6/2021 and aimed to determine the causative organism(s).
METHODS
A multiplex polymerase chain reaction (PCR) assay which enabled rapid detection of a variety of causative microorganisms of respiratory tract infections was used to analyse 13 nasopharyngeal swabs collected during the outbreak. Infection Prevention and control measures were implemented to prevent further spread of infection.
RESULTS
All 13 samples were positive for PIV-3 infection. 2 of the 13 samples were also positive for rhinovirus/enterovirus and 1 sample was also positive rhinovirus/enterovirus and coronavirus NL 63. No samples were positive for SARS-CoV-2.
DISCUSSION
Children in school settings are especially vulnerable to respiratory viral infections, including COVID-19. Children under two years are unable to wear masks reliably, and the COVID-19 vaccine was approved only for older children. Multiplex PCR assays can be used for the rapid diagnosis of respiratory infections.
CONCLUSION
We identified an outbreak of PIV-3 in a nursery school during the COVID-19 pandemic. The investigation of the outbreak highlighted that it was important not to overlook other respiratory infections including PIV-3 during the COVID-19 pandemic. The multiplex PCR assay provided rapid and accurate diagnosis of the causative organisms in the outbreak and helped to direct appropriate interventions to control the outbreak.
引言
虽然已有儿童感染3型副流感病毒（PIV - 3）暴发的报道，但据我们所知，幼儿园尚未有此类报道。由于PIV - 3感染的症状与新冠病毒（COVID - 19）感染的症状相似，因此在COVID - 19大流行期间，准确诊断PIV - 3和其他呼吸道病毒非常重要。
目的
2021年5月29日至2021年6月13日，日本宫城县一所幼儿园暴发了上呼吸道症状疫情，我们旨在确定致病病原体。
方法
采用一种能够快速检测多种呼吸道感染致病微生物的多重聚合酶链反应（PCR）检测方法，对疫情期间采集的13份鼻咽拭子进行分析。实施了感染预防和控制措施以防止感染进一步传播。
结果
所有13份样本的PIV - 3感染检测均呈阳性。13份样本中有2份同时对鼻病毒/肠道病毒检测呈阳性，1份样本同时对鼻病毒/肠道病毒和冠状病毒NL63检测呈阳性。没有样本的严重急性呼吸综合征冠状病毒2（SARS-CoV-2）检测呈阳性。
讨论
学校环境中的儿童尤其容易受到包括COVID - 19在内的呼吸道病毒感染。两岁以下的儿童无法可靠地佩戴口罩，而COVID - 19疫苗仅被批准用于年龄较大的儿童。多重PCR检测可用于呼吸道感染的快速诊断。
结论
我们在COVID - 19大流行期间发现一所幼儿园暴发了PIV - 3疫情。此次疫情调查凸显出，在COVID - 19大流行期间，不忽视包括PIV - 3在内的其他呼吸道感染非常重要。多重PCR检测为疫情中的致病病原体提供了快速准确的诊断，并有助于指导采取适当干预措施来控制疫情。
Zotero 插件