Alangari Abdulaziz, Jaffal Ahmad Abu, Alyousef Abdulaziz M, Alyousef Abdullah A
Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, P.O. Box 10219, Riyadh 11433, Saudi Arabia.
Department of Clinical Laboratory Sciences, King Saud Bin Abdulaziz University for Health Sciences, Riyadh, Saudi Arabia.
J Trop Med. 2022 May 30;2022:1745835. doi: 10.1155/2022/1745835. eCollection 2022.
The prevalence of bloodstream infections caused by extraintestinal pathogenic (ExPEC) has increased substantially. E. ST131 is one of the dominant ExPEC clones among E. bacteremia population. Metabolism can trigger the pathogenesis of some bacterial isolates, and here we evaluated and compared the metabolic traits of E. bacteremia isolates including -lactamase (BL)/extended-spectrum -lactamase (ESBL)-positive and ESBL-negative isolates and ST131 and non-ST131 isolates.
The metabolic profiles of thirty E. isolates, obtained from blood samples for hospitalized individuals at a tertiary healthcare facility in Riyadh, were determined using HiMedia carbohydrate test strips. The difference in the utilization ability between isolate groups was then statistically assessed.
Our data found that non-BL/ESBL producers were of low metabolic capacity compared with ESBL-positive isolates although the difference remained insignificant. Higher levels of utilization for some carbohydrates, such as fructose and trehalose, were detected among ST131 isolates when compared with non-ST131, and ST131 was also significantly associated with metabolizing rhamnose. The mean bio-score of both isolate groups was insignificant. We showed no link between metabolism and antimicrobial susceptibility profiles among tested blood isolates.
ST131 blood isolates were slightly higher in their carbohydrate utilization activity than non-ST131. More importantly, ST131 isolates were significantly capable of metabolizing rhamnose. Future research should focus on the factors that might drive the success of major ExPEC clones such as ST131.
由肠外致病性大肠杆菌（ExPEC）引起的血流感染患病率大幅上升。E. ST131是大肠杆菌菌血症群体中占主导地位的ExPEC克隆之一。代谢可引发某些细菌分离株的发病机制，在此我们评估并比较了大肠杆菌菌血症分离株的代谢特征，包括产β-内酰胺酶（BL）/超广谱β-内酰胺酶（ESBL）阳性和ESBL阴性分离株以及ST131和非ST131分离株。
使用HiMedia碳水化合物测试条测定从利雅得一家三级医疗机构住院患者血样中获得的30株大肠杆菌分离株的代谢谱。然后对分离株组之间的利用能力差异进行统计学评估。
我们的数据发现，与ESBL阳性分离株相比，非BL/ESBL产生菌的代谢能力较低，尽管差异不显著。与非ST131相比，在ST131分离株中检测到对某些碳水化合物（如果糖和海藻糖）的利用水平更高，并且ST131与鼠李糖代谢也显著相关。两个分离株组的平均生物得分无显著差异。我们发现受试血样分离株的代谢与抗菌药物敏感性谱之间没有关联。
ST131血样分离株的碳水化合物利用活性略高于非ST131。更重要的是，ST131分离株能够显著代谢鼠李糖。未来的研究应关注可能推动ST131等主要ExPEC克隆成功的因素。
