Pinilla Cristian Mauricio Barreto, Stincone Paolo, Brandelli Adriano
Centro de Tecnologia de Lacticínios (TECNOLAT), Instituto de Tecnologia de Alimentos, Campinas, SP 13070-178, Brazil.
CMFI Cluster of Excellence, Interfaculty Institute of Microbiology and Medicine, University of Tübingen, Tübingen 72076, Germany.
Data Brief. 2022 May 31;43:108343. doi: 10.1016/j.dib.2022.108343. eCollection 2022 Aug.
The cellular proteins of exposed to free and liposome-encapsulated nisin at sublethal concentration were hydrolyzed by trypsin and examined by tandem mass spectrometry (MS/MS) to obtain proteomic data. In the present study, we use the STRING v11.05 database analyze the interactions among the 78 upregulated proteins from obtained after treatment with sublethal concentrations of free and nanoliposome-encapsulated nisin. As result, from the upregulated proteins by free nisin was determined a network with 140 edges with two relevant nodes, containing ribosomal proteins and transmembrane transport proteins (SecD and ABC transport system). These two sets of proteins present biological connection as a group, with strong interactions and are related to detoxification and other response mechanisms. In addition, a high amount of membrane proteins was identified in the free nisin treatment. On the other hand, in the interaction analysis of upregulated proteins by liposome-loaded nisin, was found 156 edges with a single protein network, the same observed in free nisin, related to ribosomal proteins. Therefore, according with this analysis, the encapsulation of nisin into liposomes cause upregulation of ribosomal and decrease of response proteins as compared with free nisin.
将处于亚致死浓度的游离和脂质体包裹的乳链菌肽处理后的细胞蛋白质用胰蛋白酶水解，并用串联质谱（MS/MS）进行检测以获得蛋白质组学数据。在本研究中，我们使用STRING v11.05数据库分析了用亚致死浓度的游离和纳米脂质体包裹的乳链菌肽处理后获得的78种上调蛋白之间的相互作用。结果，从游离乳链菌肽上调的蛋白质中确定了一个具有140条边的网络，有两个相关节点，包含核糖体蛋白和跨膜转运蛋白（SecD和ABC转运系统）。这两组蛋白质作为一个整体呈现出生物学联系，具有强烈的相互作用，并且与解毒和其他应激反应机制相关。此外，在游离乳链菌肽处理中鉴定出大量膜蛋白。另一方面，在脂质体包裹的乳链菌肽上调蛋白的相互作用分析中，发现了一个具有156条边的单一蛋白质网络，与游离乳链菌肽中观察到的相同，与核糖体蛋白相关。因此，根据该分析，与游离乳链菌肽相比，将乳链菌肽包裹在脂质体中会导致核糖体上调和应激反应蛋白减少。
