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使用皮克林冲头从乌木(Bakh.)中采集木芯样本用于多用途分析。

Collecting wood core samples from Macassar ebony ( Bakh.) for multi-purpose analysis using pickering punch.

作者信息

Siregar Iskandar Zulkarnaen, Majiidu Muhammad, Dwiyanti Fifi Gus, Harnelly Essy, Damayanti Ratih, Karlinasari Lina, Rafi Mohamad, Septaningsih Dewi Anggraini, Parker-Forney Meaghan

机构信息

Advanced Research Laboratory, IPB University, Bogor, Indonesia.

Department of Silviculture, Faculty of Forestry and Environment, IPB University, Bogor, Indonesia.

出版信息

MethodsX. 2022 May 14;9:101728. doi: 10.1016/j.mex.2022.101728. eCollection 2022.

DOI:10.1016/j.mex.2022.101728
PMID:35677845
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9168166/
Abstract

Sample collection activities for a study of population genetics across the natural distribution of targeted tree species require a lot of resources, mainly if repeated field visits are necessary. Conventionally, population genetic studies use good sample material like leaves. In addition, cambium or small pieces of fresh wood can be used to replace leaf samples. Currently, restrictions from the permit regulation have caused only a limited number of samples that can be collected. Therefore, efficient use of samples must be designed to maximize their uses for research. Due to the small amount of successfully sampled materials, hence there are limitations to extend their uses for other analyses and are often sufficient only for genetic analysis. Therefore, innovation in sampling methods using pickering punch (https://www.agroisolab.com/pickering-punch) to collect ebony wood cores in this study is required to cover multi-analyses not only limited to genetics but also for other analyses such as isotopes, near-infrared spectroscopy (NIRs), anatomy, and chemical compounds.•Pickering punch is recommended for efficient wood core sample collection from ebony standing trees.•323 wood core samples were successfully collected from 16 natural populations across Celebes (Sulawesi).•Multi-analyses studies on sampled wood cores are possible for ebony wood identification (e.g., species and origin/provenance).

摘要

针对目标树种自然分布范围内的群体遗传学研究开展样本采集活动需要大量资源,尤其是在需要进行多次实地考察的情况下。传统上,群体遗传学研究使用树叶等优质样本材料。此外,形成层或小块新鲜木材也可用于替代树叶样本。目前,许可规定的限制导致只能采集有限数量的样本。因此,必须设计高效利用样本的方法,以最大限度地发挥其研究用途。由于成功采样的材料数量较少,因此将其用于其他分析存在局限性,通常仅足以进行遗传分析。因此,本研究需要创新采样方法,使用皮克林冲孔器(https://www.agroisolab.com/pickering-punch)采集乌木木材芯,以涵盖不仅限于遗传学的多分析,还包括同位素、近红外光谱(NIRs)、解剖学和化合物等其他分析。

•推荐使用皮克林冲孔器从乌木立木中高效采集木材芯样本。

•从苏拉威西岛的16个自然种群中成功采集了323个木材芯样本。

•对采样的木材芯进行多分析研究对于乌木识别(如物种和产地/种源)是可行的。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bdf5/9168166/f4066adca252/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bdf5/9168166/6d59aadc9436/ga1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bdf5/9168166/661b210e8610/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bdf5/9168166/e53cbdbf5578/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bdf5/9168166/16edd856f208/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bdf5/9168166/f4066adca252/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bdf5/9168166/6d59aadc9436/ga1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bdf5/9168166/661b210e8610/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bdf5/9168166/e53cbdbf5578/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bdf5/9168166/16edd856f208/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bdf5/9168166/f4066adca252/gr4.jpg

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本文引用的文献

1
Generating long-read sequences using Oxford Nanopore Technology from Diospyros celebica genomic DNA.利用 Oxford Nanopore Technology 从滇枣基因组 DNA 生成长读序列。
BMC Res Notes. 2021 Feb 27;14(1):75. doi: 10.1186/s13104-021-05484-0.
2
A simulation study of sample size for DNA barcoding.DNA条形码样本量的模拟研究。
Ecol Evol. 2015 Dec 1;5(24):5869-79. doi: 10.1002/ece3.1846. eCollection 2015 Dec.