Department of Silviculture, Faculty of Forestry and Environment, IPB University (Bogor Agricultural University), Bogor, Indonesia.
Molecular Science Research Group, Advanced Research Laboratory, IPB University (Bogor Agricultural University), Bogor, Indonesia.
BMC Res Notes. 2021 Feb 27;14(1):75. doi: 10.1186/s13104-021-05484-0.
Development of sequencing technology has opened up vast opportunities for tree genomic research in the tropics. One of the aforesaid technologies named ONT (Oxford Nanopore Technology) has attracted researchers in undertaking testings and experiments due to its affordability and accessibility. To the best of our knowledge, there has been no published reports on the use of ONT for genomic analysis of Indonesian tree species. This progress is promising for further improvement in order to acquire more genomic data for research purposes. Therefore, the present study was carried out to determine the effectiveness of ONT in generating long-read DNA sequences using DNA isolated from leaves and wood cores of Macassar ebony (Diospyros celebica Bakh.).
Long-read sequences data of leaves and wood cores of Macassar ebony were generated by using the MinION device and MinKnow v3.6.5 (ONT). The obtained data, as the first long-read sequence dataset for Macassar ebony, is of great importance to conserve the genetic diversity, understanding the molecular mechanism, and sustainable use of plant genetic resources for downstream applications.
测序技术的发展为热带树木基因组研究开辟了广阔的机会。其中一种名为 ONT（牛津纳米孔技术）的技术由于其价格合理且易于使用，吸引了研究人员进行测试和实验。据我们所知，目前还没有关于 ONT 用于印度尼西亚树种基因组分析的出版物。这一进展很有希望进一步改进，以便为研究目的获得更多的基因组数据。因此，本研究旨在确定使用 MinION 设备和 MinKnow v3.6.5（ONT）从乌木（Diospyros celebica Bakh.）叶片和木芯中分离的 DNA 生成长读 DNA 序列的 ONT 的有效性。
使用 MinION 设备和 MinKnow v3.6.5（ONT）生成了乌木叶片和木芯的长读序列数据。该数据集是首个关于乌木的长读序列数据集，对于保护遗传多样性、理解分子机制以及可持续利用植物遗传资源用于下游应用具有重要意义。
