Silvestris Erica, Minoia Carla, Guarini Attilio, Opinto Giuseppina, Negri Antonio, Dellino Miriam, Tinelli Raffaele, Cormio Gennaro, Paradiso Angelo Virgilio, De Palma Giuseppe
Gynecologic Oncology Unit, IRCCS Istituto Tumori "Giovanni Paolo II", 70124 Bari, Italy.
Haematology Unit, IRCCS Istituto Tumori "Giovanni Paolo II", 70124 Bari, Italy.
Curr Issues Mol Biol. 2022 May 19;44(5):2309-2320. doi: 10.3390/cimb44050157.
Cancer treatment related infertility (CTRI) affects more than one third of young women undergoing anti-cancer protocols, inducing a premature exhaustion of the ovarian reserve. In addition to ovarian suppression by GnRHa, oocyte and cortex cryopreservation has gained interest in patients with estrogen-sensitive tumors for whom the hormonal burst to prompt the multiple follicular growth could provide a further pro-life tumor pulsing. On the other hand, cortex reimplantation implies a few drawbacks due to the unknown consistency of the follicles to be reimplanted or the risk of reintroducing malignant cells. The capability of ovarian stem cells (OCSs) from fresh ovarian cortex fragments to differentiate in vitro to mature oocytes provides a tool to overcome these drawbacks. In fact, since ovarian cortex sampling and cryopreservation is practicable before gonadotoxic treatments, the recruitment of OSCs from defrosted fragments could provide a novel opportunity to verify their suitability to be expanded in vitro as oocyte like cells (OLCs). Here, we describe in very preliminary experiments the consistency of an OSC population from a single cryopreserved ovarian cortex after thawing as well as both their viability and their suitability to be further explored in their property to differentiate in OLCs, thus reinforcing interest in stemness studies in the treatment of female CTRI.
癌症治疗相关不孕症（CTRI）影响超过三分之一接受抗癌方案的年轻女性，导致卵巢储备过早耗尽。除了促性腺激素释放激素激动剂（GnRHa）抑制卵巢外，卵母细胞和皮质冷冻保存对于雌激素敏感肿瘤患者也受到关注，因为促使多个卵泡生长的激素激增可能会进一步促进肿瘤生长。另一方面，皮质再植入存在一些缺点，因为待再植入卵泡的一致性未知，或者存在重新引入恶性细胞的风险。来自新鲜卵巢皮质碎片的卵巢干细胞（OCS）在体外分化为成熟卵母细胞的能力提供了一种克服这些缺点的工具。事实上，由于在性腺毒性治疗前进行卵巢皮质采样和冷冻保存是可行的，从解冻碎片中招募OCS可以提供一个新的机会来验证它们作为卵母细胞样细胞（OLC）在体外扩增的适用性。在这里，我们在非常初步的实验中描述了单个冷冻保存的卵巢皮质解冻后OCS群体的一致性，以及它们的活力和进一步探索其分化为OLC特性的适用性，从而增强了对女性CTRI治疗中干细胞研究的兴趣。
